KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.224 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Lissachatina fulica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, SDS-PAGE | Lissachatina fulica |
220000 | - |
gel filtration | Lissachatina fulica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside Rb1 + 3 H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | - |
? | |
ginsenoside Rb2 + 2 H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | - |
? | |
ginsenoside Rb3 + H2O | Lissachatina fulica | - |
20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | - |
? | |
ginsenoside Rc + H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lissachatina fulica | - |
China white jade snail | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Lissachatina fulica |
Purification (Comment) | Organism |
---|---|
native enzyme 626fold from acetone powder of viscera by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and two steps of gel filtration | Lissachatina fulica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Lissachatina fulica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
162.8 | - |
purified enzyme | Lissachatina fulica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Lissachatina fulica | 2-nitrophenol + beta-D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | high activity | Lissachatina fulica | 4-methylumbelliferol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | high activity | Lissachatina fulica | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? | |
ginsenoside Rb1 + 3 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | - |
? | |
ginsenoside Rb1 + 3 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | i.e. compound K | ? | |
ginsenoside Rb2 + 2 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | - |
? | |
ginsenoside Rb2 + 2 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | i.e. compound Y | ? | |
ginsenoside Rb3 + H2O | - |
Lissachatina fulica | 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | - |
? | |
ginsenoside Rb3 + H2O | - |
Lissachatina fulica | 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | i.e. compound Mx | ? | |
ginsenoside Rc + H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
ginsenoside Rc + H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | i.e. compound Mc | ? | |
additional information | substrate specificity with various 4-nitrophenol glycosides, overview, poor activity with lactose, sucrose, trehalose, and ginsenoside Rd, the enzyme can cleave both beta-1,2-glucosidic linkage at 3-C and beta-1,6-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb1, Rb2, Rb3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively | Lissachatina fulica | ? | - |
? | |
sophorose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, SDS-PAGE | Lissachatina fulica |
Synonyms | Comment | Organism |
---|---|---|
ginsenoside-hydrolyzing beta-D-glucosidase | - |
Lissachatina fulica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lissachatina fulica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for 8 h | Lissachatina fulica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Lissachatina fulica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 30°C, stable for 24 h | Lissachatina fulica |