KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
ginsenoside Rb1 | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) | |
0.111 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) | |
0.805 | - |
ginsenoside Rd | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
3 * 102000, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
305000 | - |
gel filtration | Paecilomyces sp. (in: Eurotiomycetes) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces sp. (in: Eurotiomycetes) | - |
- |
- |
Paecilomyces sp. (in: Eurotiomycetes) 229 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 144fold by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on microcrystalline cellulose, to homogeneity, method optimization, overview | Paecilomyces sp. (in: Eurotiomycetes) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56.7 | - |
purified enzyme, substrate ginsenoside Rb1 | Paecilomyces sp. (in: Eurotiomycetes) |
129.4 | - |
purified enzyme, substrate ginsenoside Rd | Paecilomyces sp. (in: Eurotiomycetes) |
170.5 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Paecilomyces sp. (in: Eurotiomycetes) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) 229 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) | beta-D-glucose + alpha-D-glucose | - |
? | |
cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 229 | beta-D-glucose + alpha-D-glucose | - |
? | |
cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | beta-D-glucose + alpha-D-glucose | - |
? | |
gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) 229 | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 2 beta-D-glucose | - |
? | |
ginsenoside Rb1 + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
ginsenoside Rd + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) 229 | ? | - |
? | |
additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | ? | - |
? | |
sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) | 2 beta-D-glucose | - |
? | |
sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 229 | 2 beta-D-glucose | - |
? | |
sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 102000, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified enzyme, 4 h, pH 3.5, stable up to, rapid decrease in activity above | Paecilomyces sp. (in: Eurotiomycetes) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | rapid decrease in activity below pH 3.0 and above pH 4.0 | Paecilomyces sp. (in: Eurotiomycetes) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | purified enzyme, 4 h, 45°C, stable | Paecilomyces sp. (in: Eurotiomycetes) |