Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, display of the enzyme on the cell surface of Saccharomyces cerevisiae | Aspergillus oryzae |
Protein Variants | Comment | Organism |
---|---|---|
G294A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294C | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294D | site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme | Aspergillus oryzae |
G294E | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294F | site-directed mutagenesis, the mutant shows 1.5fold higher activities for substrate recognition than the wild-type enzyme | Aspergillus oryzae |
G294H | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294I | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294K | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294L | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294M | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294N | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294P | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294Q | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294R | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294S | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294T | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294V | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Aspergillus oryzae |
G294W | site-directed mutagenesis, the mutant shows 1.5fold higher activities for substrate recognition than the wild-type enzyme | Aspergillus oryzae |
G294Y | site-directed mutagenesis, the mutant shows 1.6fold higher activities for substrate recognition than the wild-type enzyme | Aspergillus oryzae |
additional information | rapid construction of a library of mutant BGL1 from Aspergillus oryzae by yeast cell surface engineering | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Aspergillus oryzae | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
celloheptaose + 6 H2O = 7 beta-D-glucose | W293 of the SDW segment, which is the residue next to the nucleophile D292 in family 3 BGL, is very important for hydrolytic reaction as a binder to a substrate. G294 of the SDWG sequence might play an important role in catalysis, interaction between the sugar rings and aromatic ring of W293 at the entrance of the catalytic pocket enhances the substrate recognition of beta-glucosidase | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + 2 H2O | - |
Aspergillus oryzae | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Aspergillus oryzae | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | interaction between the sugar rings and aromatic ring of W293 at the entrance of the catalytic pocket enhances the substrate recognition of beta-glucosidase | Aspergillus oryzae | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase 1 | - |
Aspergillus oryzae |
BGL1 | - |
Aspergillus oryzae |
More | the enzyme belongs to the glycosyl hydrolase family 3 | Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Aspergillus oryzae |