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Literature summary for 3.2.1.21 extracted from

  • Boonclarm, D.; Sornwatana, T.; Arthan, D.; Kongsaeree, P.; Svasti, J.
    beta-Glucosidase catalyzing specific hydrolysis of an iridoid beta-glucoside from Plumeria obtusa (2006), Acta Biochim. Biophys. Sin., 38, 563-570.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
4-chloromercuribenzoate 9% activation at 1 mM Plumeria obtusa
Ca2+ 15% activation at 1 mM Plumeria obtusa
EDTA 10% activation at 1 mM Plumeria obtusa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 21% inhibition at 1 mM Plumeria obtusa
D-glucono-1,5-lactone 44% inhibition at 1 mM, 91% at 10 mM Plumeria obtusa
Fe3+ slight inhibition of 8% at 1 mM Plumeria obtusa
Hg2+ 14% inhibition at 1 mM Plumeria obtusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
plumieride coumarate beta-D-glucoside pH 5.5, 37°C Plumeria obtusa
5.04
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.5, 37°C Plumeria obtusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration Plumeria obtusa
60600
-
1 * 60600, SDS-PAGE Plumeria obtusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plumieride coumarate beta-D-glucoside + H2O Plumeria obtusa an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, the Plumeria obtusa beta-glucosidase shows high specificity for its natural substrate, plumieride coumarate glucoside 13-O-coumarylplumieride + beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Plumeria obtusa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Plumeria obtusa

Purification (Commentary)

Purification (Comment) Organism
native enzyme 217fold from flower to homogeneity by anion exchange and hydrophobic interaction chromatography, gel filtration, concanavalin A affinity chromatography and again anion exchange chromatography Plumeria obtusa

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Plumeria obtusa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.38
-
purified enzyme Plumeria obtusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O
-
Plumeria obtusa 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Plumeria obtusa 4-nitrophenol + beta-D-glucose
-
?
additional information substrate specificity, overview, poor hydrolysis of dalcochinin-8'-O-beta-glucoside, 4-methylumbelliferyl-beta-glucoside, and esculin, and no hydrolysis of alkyl-beta-glucosides, glucobioses, cyanogenic-beta-glucosides, steroid beta-glucosides, nor other iridoid beta-glucosides, including gonocaryoside A, or its derivative kingiside Plumeria obtusa ?
-
?
plumieride coumarate beta-D-glucoside + H2O an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, the Plumeria obtusa beta-glucosidase shows high specificity for its natural substrate, plumieride coumarate glucoside Plumeria obtusa 13-O-coumarylplumieride + beta-D-glucose
-
?
plumieride coumarate beta-D-glucoside + H2O an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, substrate and product identification and structure determination by NMR, overview Plumeria obtusa 13-O-coumarylplumieride + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60600, SDS-PAGE Plumeria obtusa

Synonyms

Synonyms Comment Organism
iridoid beta-glucoside
-
Plumeria obtusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Plumeria obtusa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.9
-
plumieride coumarate beta-D-glucoside pH 5.5, 37°C Plumeria obtusa
30.7
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.5, 37°C Plumeria obtusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Plumeria obtusa

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 75% of maximal activity within the range of pH 4.5-6.5 Plumeria obtusa

pI Value

Organism Comment pI Value Maximum pI Value
Plumeria obtusa isoelectric focusing
-
4.9