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Literature summary for 3.2.1.21 extracted from

  • Mase, T.; Mori, S.; Yokoe, M.
    Purification, characterization, and a potential application of beta-glucosidase from Aspergillus pulverulentus YM-80 (2004), J. Appl. Glycosci., 51, 211-216.
No PubMed abstract available
Search for more literature for 3.2.1.21

Application

Application Comment Organism
nutrition the enzyme is useful in tofu production where it hydrolyses isoflavone glucosides allowing the preparation of aglycone isoflavone-enriched tofu Aspergillus pulverulentus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition Aspergillus pulverulentus
SDS complete inhibition Aspergillus pulverulentus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 2 * 118000, SDS-PAGE Aspergillus pulverulentus
240000
-
 gel filtration Aspergillus pulverulentus

Organism

Organism UniProt Comment Textmining
Aspergillus pulverulentus
-
 isolated from soil
-
Aspergillus pulverulentus YM-80
-
 isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
18fold by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration to homogeneity Aspergillus pulverulentus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
308
-
 purified enzyme Aspergillus pulverulentus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active with a broad range of beta-linked glucosides, but not with alpha-linked saccharides, the enzyme hydrolyses some isoflavones to aglycone and beta-D-glucose, but shows no hydrolysis of isoflavones with 6''-O-acetyl and 6''-O-malonyl glucosides Aspergillus pulverulentus ?
-
 ?
additional information the enzyme is active with a broad range of beta-linked glucosides, but not with alpha-linked saccharides, the enzyme hydrolyses some isoflavones to aglycone and beta-D-glucose, but shows no hydrolysis of isoflavones with 6''-O-acetyl and 6''-O-malonyl glucosides Aspergillus pulverulentus YM-80 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 118000, SDS-PAGE Aspergillus pulverulentus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus pulverulentus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Aspergillus pulverulentus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Aspergillus pulverulentus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 stable Aspergillus pulverulentus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus pulverulentus
-
-
 4.5