Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is unaffected by EDTA and Tween 20 | Volvariella volvacea | |
Triton X-100 | - |
Volvariella volvacea |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Volvariella volvacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition | Volvariella volvacea | |
Hg2+ | complete inhibition | Volvariella volvacea | |
additional information | the enzyme is unaffected by EDTA and Tween 20 | Volvariella volvacea | |
Ni2+ | - |
Volvariella volvacea | |
SDS | complete inhibition | Volvariella volvacea | |
Zn2+ | - |
Volvariella volvacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-nitrophenyl beta-D-glucoside | pH 6.4, 50°C, recombinant enzyme | Volvariella volvacea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is unaffected by Mn2+, Mg2+, and Co2+ | Volvariella volvacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
4 * 97000, recombinant enzyme, SDS-PAGE | Volvariella volvacea |
380000 | - |
recombinant enzyme, gel filtration | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | Q9C3Z9 | - |
- |
Volvariella volvacea V1-1 | Q9C3Z9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 16fold to homogeneity by ion exchange chromatography and gel filtration | Volvariella volvacea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96 | - |
purified recombinant enzyme | Volvariella volvacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Volvariella volvacea | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Volvariella volvacea V1-1 | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Volvariella volvacea | ? | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Volvariella volvacea V1-1 | ? | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Volvariella volvacea | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Volvariella volvacea V1-1 | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Volvariella volvacea | beta-D-glucose + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Volvariella volvacea V1-1 | beta-D-glucose + beta-D-glucose | - |
? | |
additional information | no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside | Volvariella volvacea | ? | - |
? | |
additional information | no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside | Volvariella volvacea V1-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 97000, recombinant enzyme, SDS-PAGE | Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Volvariella volvacea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant enzyme, half-life 1 h | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Volvariella volvacea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8 | stable, purified recombinant enzyme | Volvariella volvacea |