Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is induced by growth on rice bran hemicellulose, and slightly by pectin, xylan, and glucose, no effect by EDTA, L-fucose, G-glucuronic acid, D-mannose, L-rhamnose, and D-xylose | Paenibacillus sp. |
Application | Comment | Organism |
---|---|---|
nutrition | the enzyme is useful in rice polishing by degradation and assimilation of rice bran hemicellulose of the outer grain skin | Paenibacillus sp. |
Cloned (Comment) | Organism |
---|---|
gene bglA, DNA and amino acid sequence determination and analysis, cDNA library construction, expression in Escherichi coli | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 12% inhibition at 1 mM | Paenibacillus sp. | |
Cu2+ | 79% inhibition at 1 mM | Paenibacillus sp. | |
D-galactose | 46% inhibition at 1.5 mM | Paenibacillus sp. | |
D-glucose | 76% inhibition at 1.5 mM | Paenibacillus sp. | |
D-glucuronic acid | 12% inhibition at 1.5 mM | Paenibacillus sp. | |
D-mannose | 10% inhibition at 1.5 mM | Paenibacillus sp. | |
Fe2+ | 63% inhibition at 1 mM | Paenibacillus sp. | |
Hg2+ | 99% inhibition at 1 mM | Paenibacillus sp. | |
Mn2+ | 40% inhibition at 1 mM | Paenibacillus sp. | |
additional information | no effect by EDTA, DTT, 2-mercaptoethanol, iodoacetc acid, glutathione, L-fucose, L-rhamnose, and D-xylose | Paenibacillus sp. | |
NEM | 26% inhibition at 1 mM | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.0, 30°C, recombinant enzyme | Paenibacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Paenibacillus sp. | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 14% activation at 1 mM | Paenibacillus sp. | |
additional information | the enzyme is not affected by Mg2+ | Paenibacillus sp. | |
Zn2+ | 11% activation at 1 mM | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
recombinant enzyme, gel filtration | Paenibacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rice bran hemicellulose + H2O | Paenibacillus sp. | the enzyme is involved in degradation | ? | - |
? | |
rice bran hemicellulose + H2O | Paenibacillus sp. HC1 | the enzyme is involved in degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | Q2WGB4 | isolated from soil, gene bglA | - |
Paenibacillus sp. HC1 | Q2WGB4 | isolated from soil, gene bglA | - |
Purification (Comment) | Organism |
---|---|
recombinant BglA 52.9fold from Escherichia coli by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.4 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, enzyme expression induced by growth on rice bran hemicellulose | Paenibacillus sp. |
74.6 | - |
purified enzyme | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Paenibacillus sp. | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Paenibacillus sp. | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Paenibacillus sp. HC1 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Paenibacillus sp. | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Paenibacillus sp. HC1 | 4-nitrophenol + beta-D-glucose | - |
? | |
additional information | substrate specificity, overview | Paenibacillus sp. | ? | - |
? | |
additional information | substrate specificity, overview | Paenibacillus sp. HC1 | ? | - |
? | |
rice bran hemicellulose + H2O | degradation | Paenibacillus sp. | ? | - |
? | |
rice bran hemicellulose + H2O | the enzyme is involved in degradation | Paenibacillus sp. | ? | - |
? | |
rice bran hemicellulose + H2O | degradation | Paenibacillus sp. HC1 | ? | - |
? | |
rice bran hemicellulose + H2O | the enzyme is involved in degradation | Paenibacillus sp. HC1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, recombinant enzyme, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
BglA | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, loss of 50% activity | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Paenibacillus sp. |