Any feedback?
Literature summary for 3.2.1.21 extracted from
- Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii beta-glucosidase II with high hydrolytic activity on sophorose (2005), Appl. Microbiol. Biotechnol., 66, 527-535.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme might be useful as biocatalyst in the synthesis of glyco-conjugates, overview | Schwanniomyces etchellsii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain JM109 | Schwanniomyces etchellsii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | binds to the active site, 4-nitrophenyl beta-D-glucoside partially protects against inactivation | Schwanniomyces etchellsii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
2 * 83000, recombinant enzyme | Schwanniomyces etchellsii |
| 176000 | - |
recombinant enzyme | Schwanniomyces etchellsii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schwanniomyces etchellsii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain JM109 | Schwanniomyces etchellsii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| celloheptaose + 6 H2O = 7 beta-D-glucose | the enzyme contains a His residue which is important for catalytic activity | Schwanniomyces etchellsii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.77 | - |
purified recombinant enzyme, substrate sophorose | Schwanniomyces etchellsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Schwanniomyces etchellsii | 4-nitrophenol + beta-D-glucose | - |
? | |
| amygdalin + H2O | - |
Schwanniomyces etchellsii | ? | - |
? | |
| additional information | the enzyme shows broad substrate specificity hydrolyzing beta-linked gluco-disaccharides and oligosaccharides, and transglycosylation activity forming beta-linked di- and oligosaccharides | Schwanniomyces etchellsii | ? | - |
? | |
| salicin + H2O | - |
Schwanniomyces etchellsii | ? | - |
? | |
| sophorose + H2O | - |
Schwanniomyces etchellsii | 2 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 83000, recombinant enzyme | Schwanniomyces etchellsii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase II | - |
Schwanniomyces etchellsii |
| More | the enzyme belongs to the glycosyl hydrolase family 3 | Schwanniomyces etchellsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is thermotolerant | Schwanniomyces etchellsii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
