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Literature summary for 3.2.1.21 extracted from

  • Marana, S.R.; Jacobs-Lorena, M.; Terra, W.R.; Ferreira, C.
    Amino acid residues involved in substrate binding and catalysis in an insect digestive beta-glycosidase (2001), Biochim. Biophys. Acta, 1545, 41-52.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Spodoptera frugiperda

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition Spodoptera frugiperda
cellopentaose competitive inhibition Spodoptera frugiperda
cellotetraose competitive inhibition Spodoptera frugiperda
cellotriose competitive inhibition Spodoptera frugiperda
decyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
delta-gluconolactone competitive inhibition Spodoptera frugiperda
heptyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
hexyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
nonyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
octyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
p-nitrophenyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda
pentyl-beta-D-glucopyranoside competitive inhibition Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.52
-
 p-nitrophenyl-beta-D-xylopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.6
-
 amygdalin 30°C, pH 6.0 Spodoptera frugiperda
0.74
-
 gentiobiose 30°C, pH 6.0 Spodoptera frugiperda
0.8
-
 p-nitrophenyl-beta-D-fucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
2.1
-
 p-nitrophenyl-beta-D-galactopyranoside 30°C, pH 6.0 Spodoptera frugiperda
2.3
-
 4-methylumbelliferyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
2.7
-
 cellobiose 30°C, pH 6.0 Spodoptera frugiperda
4
-
 octyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
 Spodoptera frugiperda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Spodoptera frugiperda 4-methylumbelliferol + beta-D-glucose
-
 ?
amygdalin + H2O
-
 Spodoptera frugiperda beta-D-glucose + ?
-
 ?
cellobiose + H2O
-
 Spodoptera frugiperda 2 beta-D-glucose
-
 ?
gentiobiose + H2O
-
 Spodoptera frugiperda beta-D-glucose + ?
-
 ?
octyl-beta-D-glucopyranoside + H2O
-
 Spodoptera frugiperda octanol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-fucoopyranoside + H2O
-
 Spodoptera frugiperda p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Spodoptera frugiperda p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Spodoptera frugiperda p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Spodoptera frugiperda p-nitrophenol + beta-D-xylose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 p-nitrophenyl-beta-D-xylopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.06
-
 octyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.17
-
 p-nitrophenyl-beta-D-galactopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.32
-
 amygdalin 30°C, pH 6.0 Spodoptera frugiperda
0.5
-
 cellobiose 30°C, pH 6.0 Spodoptera frugiperda
0.56
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
0.6
-
 gentiobiose 30°C, pH 6.0 Spodoptera frugiperda
1.73
-
 4-methylumbelliferyl-beta-D-glucopyranoside 30°C, pH 6.0 Spodoptera frugiperda
2.4
-
 p-nitrophenyl-beta-D-fucopyranoside 30°C, pH 6.0 Spodoptera frugiperda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 delta-gluconolactone vs. p-nitrophenyl-beta-D-glucopyranoside,30°C, pH 6.0 Spodoptera frugiperda
0.29
-
 cellotetraose vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
0.4
-
 p-nitrophenyl-beta-D-glucopyranoside vs. cellobiose, 30°C, pH 6.0 Spodoptera frugiperda
0.56
-
 cellopentaose vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
0.85
-
 cellotriose vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
1.4
-
 decyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
1.7
-
 nonyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
2.8
-
 cellobiose vs. p-nitrophenyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
3.1
-
 octyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
4
-
 octyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
5.9
-
 heptyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
9.8
-
 hexyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda
18
-
 pentyl-beta-D-glucopyranoside vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0 Spodoptera frugiperda