Literature summary for 3.2.1.21 extracted from

Issam, S.M.; Mohamed, G.; Dominique, L.M.; Thierry, M.; Farid, L.; Nejib, M.
A beta-glucosidase from Sclerotinia sclerotiorum: Biochemical characterization and use in oligosaccharide synthesis (2004), Appl. Biochem. Biotechnol., 112, 63-77.

Search for more literature for 3.2.1.21

Inhibitors

Inhibitors	Comment	Organism	Structure
Fe2+	strong inhibition	Sclerotinia sclerotiorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	p-nitrophenyl-beta-D-glucopyranoside	-	Sclerotinia sclerotiorum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Sclerotinia sclerotiorum	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	35% activation	Sclerotinia sclerotiorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	2 * 60000, SDS-PAGE	Sclerotinia sclerotiorum
130000	-	gel filtration	Sclerotinia sclerotiorum

Organism

Organism	UniProt	Comment	Textmining
Sclerotinia sclerotiorum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, gel filtration, anion-exchange, gel filtration	Sclerotinia sclerotiorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl-beta-D-glucopyranoside + H2O	beta-Glu x also has transglucosylation activity	Sclerotinia sclerotiorum	p-nitrophenol + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, SDS-PAGE	Sclerotinia sclerotiorum

Synonyms

Synonyms	Comment	Organism
beta-Glu x	-	Sclerotinia sclerotiorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	60	-	Sclerotinia sclerotiorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Sclerotinia sclerotiorum

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Release 2023.1 (January 2023)