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Literature summary for 3.2.1.21 extracted from
- Purification and properties of beta-glucosidase from Humicola insolvens YH-8 (1980), Agric. Biol. Chem., 44, 1729-1735.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable in the presence of dimethyl sulfoxide or ethyl alcohol | Humicola insolens |
| stable in the presence of urea | Humicola insolens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 55% of original activity inhibitited | Humicola insolens |  |
| NaCl | low activity even at low concentrations | Humicola insolens | |
| Sr2+ | slight inhibition at 10 mM | Humicola insolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Humicola insolens | |
| 0.86 | - |
Salicin | - |
Humicola insolens | |
| 1.52 | - |
cellobiose | - |
Humicola insolens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Humicola insolens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
SDS-PAGE | Humicola insolens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Humicola insolens | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humicola insolens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 1.7%, w/w, mannose, 0.8%, w/w, glucose residues | Humicola insolens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Humicola insolens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | maximal enzyme synthesis, if cells grown in wheat bran medium | Humicola insolens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
- |
Humicola insolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Humicola insolens | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Humicola insolens | beta-D-glucose + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Humicola insolens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 5 min | Humicola insolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Humicola insolens |
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