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Literature summary for 3.2.1.21 extracted from

  • Ferreira, C.; Terra, W.R.
    Physical and kinetic properties of a plasma-membrane-bound beta-D-glucosidase (cellobiase) from midgut cells of an insect (Rhynchosciara americana larva) (1983), Biochem. J., 213, 43-51.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl-alpha-D-glucoside 48.6% inhibition at 15 mM Rhynchosciara americana
D-glucono-1,5-lactone competitive inhibition, Ki: 0.0005 mM Rhynchosciara americana
gamma-galactonolactone competitive inhibition, Ki: 0.3 mM Rhynchosciara americana
maltose 44.8% inhibition at 15 mM Rhynchosciara americana
melibiose 20.2% inhibition at 15 mM Rhynchosciara americana
sucrose 51% inhibition at 15 mM Rhynchosciara americana
Tris competitive inhibition, Ki: 19 mM Rhynchosciara americana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl-beta-D-fucoside
-
 Rhynchosciara americana
0.53
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rhynchosciara americana
0.61
-
 cellobiose
-
 Rhynchosciara americana
0.8
-
 Salicin
-
 Rhynchosciara americana
0.9
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Rhynchosciara americana
3.5
-
 phenyl-beta-D-glucoside
-
 Rhynchosciara americana
5.3
-
 4-nitrophenyl-beta-D-galactoside
-
 Rhynchosciara americana

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane detergent-solubilized and papain-released forms Rhynchosciara americana 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 detergent-solubilized form, density-gradient centrifugation Rhynchosciara americana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Rhynchosciara americana cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Rhynchosciara americana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, density gradient centrifugation Rhynchosciara americana

Source Tissue

Source Tissue Comment Organism Textmining
midgut from larvae Rhynchosciara americana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Rhynchosciara americana 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Rhynchosciara americana 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O retention of configuration Rhynchosciara americana 2-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rhynchosciara americana beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Rhynchosciara americana 2 beta-D-glucose
-
 ?
phenyl-beta-D-glucoside + H2O low activity Rhynchosciara americana phenol + beta-D-glucose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 completely stable up to for 2 h Rhynchosciara americana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Rhynchosciara americana