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Literature summary for 3.2.1.21 extracted from

  • Kohchi, C.; Hayashi, M.; Nagai, S.
    Purification and properties of beta-glucosidase from Candida pelliculosa var. acetaetherius (1985), Agric. Biol. Chem., 49, 779-784.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 6.5 mM Wickerhamomyces anomalus
Co2+ 40% inhibition at 1 mM Wickerhamomyces anomalus
Cu2+ nearly complete inhibition at 1 mM Wickerhamomyces anomalus
Hg2+ complete inhibition at 1 mM Wickerhamomyces anomalus
p-chloromercuribenzoic acid strong inhibition at 1 mM Wickerhamomyces anomalus
SDS 79% inhibition at 1 mM Wickerhamomyces anomalus
Zn2+ 67% inhibition at 1 mM Wickerhamomyces anomalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Wickerhamomyces anomalus
19.2
-
 Salicin
-
 Wickerhamomyces anomalus
37
-
 cellobiose
-
 Wickerhamomyces anomalus
44.4
-
 methyl-beta-D-glucoside
-
 Wickerhamomyces anomalus
100
-
 gentiobiose
-
 Wickerhamomyces anomalus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Wickerhamomyces anomalus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 4 * 90000, SDS-PAGE Wickerhamomyces anomalus
360000
-
 gel filtration Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Wickerhamomyces anomalus cellobiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Wickerhamomyces anomalus low activity beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Wickerhamomyces anomalus gentiobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus
-
 var. acetaetherius
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Wickerhamomyces anomalus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Wickerhamomyces anomalus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Wickerhamomyces anomalus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
207
-
-
 Wickerhamomyces anomalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Wickerhamomyces anomalus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Wickerhamomyces anomalus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O low activity Wickerhamomyces anomalus 2-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O specific for beta-configuration of sugars Wickerhamomyces anomalus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Wickerhamomyces anomalus 4-nitrophenol + beta-D-xylose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Wickerhamomyces anomalus beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Wickerhamomyces anomalus beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Wickerhamomyces anomalus beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Wickerhamomyces anomalus 2 beta-D-glucose
-
 ?
gentiobiose + H2O
-
 Wickerhamomyces anomalus 2 beta-D-glucose
-
 ?
methyl-beta-D-glucoside + H2O very low activity Wickerhamomyces anomalus beta-D-glucose + methanol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 90000, SDS-PAGE Wickerhamomyces anomalus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Wickerhamomyces anomalus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Wickerhamomyces anomalus