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Literature summary for 3.2.1.21 extracted from

  • Patchett, M.L.; Daniel, R.M.; Morgan, H.W.
    Purification and properties of a stable beta-glucosidase from an extremely thermophilic anaerobic bacterium (1987), Biochem. J., 243, 779-787.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 30% stimulation at 1 mM thermophilic anaerobic bacterium
Cysteine hydrochloride slight activation at 5 mM thermophilic anaerobic bacterium
dithiothreitol slight activation at 1 mM thermophilic anaerobic bacterium
EDTA slight activation at 5 mM thermophilic anaerobic bacterium
glycerol 25% stimulation at 10%, w/v thermophilic anaerobic bacterium

General Stability

General Stability Organism
glycerol most effective in stabilizing thermophilic anaerobic bacterium
sensitive against repeated thawing and freezing thermophilic anaerobic bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM thermophilic anaerobic bacterium
Al3+ strong inhibition at 1 mM thermophilic anaerobic bacterium
Ba2+ slight inhibition at 1 mM thermophilic anaerobic bacterium
Ca2+ slight inhibition at 1 mM thermophilic anaerobic bacterium
Cd2+ strong inhibition at 1 mM thermophilic anaerobic bacterium
Co2+ nearly complete inhibition at 1 mM thermophilic anaerobic bacterium
Cu2+ complete inhibition at 1 mM thermophilic anaerobic bacterium
ethanol 20% inhibition at 5%, w/v thermophilic anaerobic bacterium
Hg2+ complete inhibition at 1 mM thermophilic anaerobic bacterium
Mn2+ slight inhibition at 1 mM thermophilic anaerobic bacterium
Ni2+ strong inhibition at 1 mM thermophilic anaerobic bacterium
p-chloromercuribenzene sulfonic acid nearly complete inhibition at 1 mM thermophilic anaerobic bacterium
SDS 87% inhibition at 1%, w/v thermophilic anaerobic bacterium
Tris inhibitory effect thermophilic anaerobic bacterium
Triton X-100 25% inhibition at 1%, w/v thermophilic anaerobic bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 thermophilic anaerobic bacterium
0.73
-
 cellobiose
-
 thermophilic anaerobic bacterium

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated
-
 thermophilic anaerobic bacterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Br- slight activation at 5 mM thermophilic anaerobic bacterium
I- 40% activation at 5 mM thermophilic anaerobic bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000 50000 gel filtration, SDS-PAGE thermophilic anaerobic bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O thermophilic anaerobic bacterium sophorose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O thermophilic anaerobic bacterium lactose beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O thermophilic anaerobic bacterium cellobiose beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r
4-O-beta-D-glucopyranosyl-D-glucose + H2O thermophilic anaerobic bacterium transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r
6-O-beta-D-glucopyranosyl-D-glucose + H2O thermophilic anaerobic bacterium low activity beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O thermophilic anaerobic bacterium gentiobiose beta-D-glucose + D-glucose
-
 ?
laminarin + H2O thermophilic anaerobic bacterium low activity beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
thermophilic anaerobic bacterium
-
 thermophilic anaerobic gram-negative bacterium
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 thermophilic anaerobic bacterium

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, preparative gel electrophoresis thermophilic anaerobic bacterium

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 thermophilic anaerobic bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
149
-
-
 thermophilic anaerobic bacterium

Storage Stability

Storage Stability Organism
4°C, 1-2% loss of activity per week thermophilic anaerobic bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity thermophilic anaerobic bacterium 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin thermophilic anaerobic bacterium 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 thermophilic anaerobic bacterium 2-nitrophenol + beta-D-galactopyranose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 thermophilic anaerobic bacterium 2-nitrophenol + D-glucopyranose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose thermophilic anaerobic bacterium beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O best substrate thermophilic anaerobic bacterium 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity thermophilic anaerobic bacterium 4-nitrophenol + beta-D-xylose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose thermophilic anaerobic bacterium beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose thermophilic anaerobic bacterium beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r
4-O-beta-D-glucopyranosyl-D-glucose + H2O transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols thermophilic anaerobic bacterium beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity thermophilic anaerobic bacterium beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose thermophilic anaerobic bacterium beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 thermophilic anaerobic bacterium 2 beta-D-glucose
-
 ?
esculin + H2O
-
 thermophilic anaerobic bacterium beta-D-glucose + 6,7-dihydroxycoumarin
-
 ?
laminarin + H2O low activity thermophilic anaerobic bacterium beta-D-glucose + ?
-
 ?
phenyl-beta-D-glucoside + H2O low activity thermophilic anaerobic bacterium phenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000-50000, SDS-PAGE thermophilic anaerobic bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 half-life: 130 min at pH 7.0 thermophilic anaerobic bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 thermophilic anaerobic bacterium