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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a beta-glucosidase (cellobiase) from a mushroom Termitomyces clypeatus (1991), Biochim. Biophys. Acta, 1076, 215-220.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | competitive inhibition, Ki: 1.7 mM | Termitomyces clypeatus |  |
| cellobiose | competitive inhibition, Ki: 1.9 mM | Termitomyces clypeatus | |
| Cu2+ | 20% inhibition at 2 mM | Termitomyces clypeatus | |
| Hg2+ | 50% inhibition at 2 mM | Termitomyces clypeatus | |
| N-bromosuccinimide | 93% inhibition at 0.5 mM | Termitomyces clypeatus | |
| p-chloromercuribenzoic acid | 70% inihibition at 0.5 mM | Termitomyces clypeatus | |
| SDS | 30% inhibition at 35 mM | Termitomyces clypeatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
cellobiose | - |
Termitomyces clypeatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Termitomyces clypeatus | - |
- |
| intracellular | present in the very early growth phase | Termitomyces clypeatus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 450000 | - |
gel filtration | Termitomyces clypeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Termitomyces clypeatus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Termitomyces clypeatus | one of the two best substrates | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Termitomyces clypeatus | low activity | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Termitomyces clypeatus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Termitomyces clypeatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 24% carbohydrate content | Termitomyces clypeatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, HPLC chromatography | Termitomyces clypeatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Termitomyces clypeatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 57.47 | - |
- |
Termitomyces clypeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Termitomyces clypeatus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Termitomyces clypeatus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | one of the two best substrates | Termitomyces clypeatus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Termitomyces clypeatus | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | one of the two best substrates | Termitomyces clypeatus | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Termitomyces clypeatus | beta-D-glucose + D-glucose | - |
? | |
| 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Termitomyces clypeatus | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Termitomyces clypeatus | 2 beta-D-glucose | - |
? | |
| methyl-beta-D-glucoside + H2O | very low activity | Termitomyces clypeatus | beta-D-glucose + methanol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Termitomyces clypeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to for 30 min | Termitomyces clypeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Termitomyces clypeatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 10 | stable within for 30 min | Termitomyces clypeatus |
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