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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a geniposide-hydrolyzing beta-glucosidase from Eubacterium sp. A-44, a strict anaerobe from human feces (1995), Biol. Pharm. Bull., 18, 1175-1178.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | complete inhibition at 0.2 mM | Eubacterium sp. |  |
| Cu2+ | strong inhibition at 0.1 mM | Eubacterium sp. | |
| nojirimycin bisulfite | strong inhibition at 0.012 mM | Eubacterium sp. | |
| p-chloromercuribenzene sulfonic acid | complete inhibition at 0.02 mM | Eubacterium sp. | |
| p-chloromercuribenzoic acid | strong inhibition at 0.1 mM | Eubacterium sp. | |
| Zn2+ | slight inhibition at 1 mM | Eubacterium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
geniposide | - |
Eubacterium sp. | |
| 0.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Eubacterium sp. | |
| 0.21 | - |
esculin | - |
Eubacterium sp. | |
| 0.22 | - |
Salicin | - |
Eubacterium sp. | |
| 0.91 | - |
cellobiose | - |
Eubacterium sp. | |
| 2.9 | - |
amygdalin | - |
Eubacterium sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Eubacterium sp. | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Eubacterium sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Eubacterium sp. | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Eubacterium sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Eubacterium sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.7 | - |
for 4-nitrophenyl-beta-D-glucopyranoside | Eubacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Eubacterium sp. | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O | amygdalin | Eubacterium sp. | HCN + ? | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Eubacterium sp. | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Eubacterium sp. | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Eubacterium sp. | 2 beta-D-glucose | - |
? | |
| esculin + H2O | - |
Eubacterium sp. | beta-D-glucose + 6,7-dihydroxycoumarin | - |
? | |
| geniposide + H2O | - |
Eubacterium sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE | Eubacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Eubacterium sp. |
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