Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Riou, C.; Salmon, J.P.; Vallier, M.J.; Gunata, Z.; Barre, P.
    Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae (1998), Appl. Environ. Microbiol., 64, 3607-3614.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ethanol 30% stimulation at 15%, v/v , 15% stimulation at 20%, v/v Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ nearly complete inhibition at 5 mM Aspergillus oryzae
beta-D-glucose competitive inhibition, Ki: 1.36 mM Aspergillus oryzae
castanospermine nearly complete inhibition at 10 mM Aspergillus oryzae
Cu2+ nearly complete inhibition at 5 mM Aspergillus oryzae
D-glucono-1,5-lactone competitive inhibition, Ki: 12.5 mM Aspergillus oryzae
diethyldicarbonate nearly complete inhibition at 10 mM Aspergillus oryzae
Fe3+ strong inhibition at 5 mM Aspergillus oryzae
Hg2+ complete inhibition at 5 mM Aspergillus oryzae
methyldeoxynojirimycin strong inhibition at 10 mM Aspergillus oryzae
N-bromosuccinimide complete inhibition at 10 mM Aspergillus oryzae
SDS strong inhibition at 10 mM Aspergillus oryzae
Zn2+ strong inhibition at 5 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
4-nitrophenyl-beta-D-glucoside
-
Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular most effective induction of enzyme synthesis in medium containing quercetin Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 77% stimulation at 5 mM Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Aspergillus oryzae low activity beta-D-glucose + D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Aspergillus oryzae sophorose beta-D-glucose + D-glucose
-
?
3-O-alpha-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae nigerose alpha-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Aspergillus oryzae laminaribiose beta-D-glucose + D-glucose
-
?
4-O-alpha-D-glucopyranosyl-D-glucose Aspergillus oryzae maltose alpha-D-glucose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Aspergillus oryzae lactose beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-xylopyranosyl-D-xylose + H2O Aspergillus oryzae
-
beta-D-xylose + D-xylose
-
?
6-O-alpha-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae isomaltose alpha-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus oryzae gentiobiose beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O Aspergillus oryzae
-
5 D-glucose
-
?
cellotetraose + 3 H2O Aspergillus oryzae
-
4 D-glucose
-
?
cellotriose + 2 H2O Aspergillus oryzae
-
3 D-glucose
-
?
laminarin + H2O Aspergillus oryzae low activity beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.07
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Aspergillus oryzae beta-D-glucose + D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Aspergillus oryzae beta-D-glucose + D-glucose
-
?
3-O-alpha-D-glucopyranosyl-D-glucose + H2O nigerose Aspergillus oryzae alpha-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Aspergillus oryzae beta-D-glucose + D-glucose
-
?
4-nitrophenyl beta-D-galactoside + H2O low activity Aspergillus oryzae 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus oryzae 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Aspergillus oryzae 4-nitrophenol + beta-D-xylose
-
?
4-O-alpha-D-glucopyranosyl-D-glucose maltose Aspergillus oryzae alpha-D-glucose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Aspergillus oryzae beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus oryzae beta-D-glucose + D-glucose
-
?
4-O-beta-D-xylopyranosyl-D-xylose + H2O
-
Aspergillus oryzae beta-D-xylose + D-xylose
-
?
6-O-alpha-D-glucopyranosyl-D-glucose + H2O isomaltose Aspergillus oryzae alpha-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Aspergillus oryzae beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Aspergillus oryzae beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O
-
Aspergillus oryzae 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Aspergillus oryzae 4 D-glucose
-
?
cellotriose + 2 H2O
-
Aspergillus oryzae 3 D-glucose
-
?
laminarin + H2O low activity Aspergillus oryzae beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
HGT-BG
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus oryzae