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Literature summary for 3.2.1.2 extracted from
- beta-Amylases from various fungal strains. A review (2004), Acta Microbiol. Immunol. Hung., 51, 85-95.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | inactivation, the enzyme can be reactivated by L-cysteine | Aspergillus nidulans |  |
| Ag+ | - |
Brettanomyces naardenensis | |
| Ag+ | - |
Phaffia rhodozyma | |
| Cu2+ | - |
Brettanomyces naardenensis | |
| Cu2+ | - |
Phaffia rhodozyma | |
| Hg2+ | - |
Brettanomyces naardenensis | |
| Hg2+ | - |
Phaffia rhodozyma | |
| additional information | no inhibition by 4-chloromercuribenzoate, heavy metal ions, nor Schardinger dextrins | Syncephalastrum racemosum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | partially cell-bound | Phaffia rhodozyma | - |
- |
| extracellular | partially cell-bound | Brettanomyces naardenensis | - |
- |
| periplasm | - |
Phaffia rhodozyma | - |
- |
| periplasm | - |
Brettanomyces naardenensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Syncephalastrum racemosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Saccharomyces cerevisiae | - |
? | - |
? | |
| starch + H2O | Aspergillus nidulans | - |
? | - |
? | |
| starch + H2O | Nakazawaea holstii | - |
? | - |
? | |
| starch + H2O | Phaffia rhodozyma | - |
? | - |
? | |
| starch + H2O | Aspergillus terreus | - |
? | - |
? | |
| starch + H2O | Saccharomyces sp. | - |
? | - |
? | |
| starch + H2O | Wickerhamomyces anomalus | - |
? | - |
? | |
| starch + H2O | Thermomyces lanuginosus | - |
? | - |
? | |
| starch + H2O | Syncephalastrum racemosum | - |
? | - |
? | |
| starch + H2O | Malbranchea cinnamomea | - |
? | - |
? | |
| starch + H2O | Trichosporon beigelii | - |
? | - |
? | |
| starch + H2O | Brettanomyces naardenensis | - |
? | - |
? | |
| starch + H2O | Corynascus sepedonium | - |
? | - |
? | |
| starch + H2O | Debaryomyces sp. | - |
? | - |
? | |
| starch + H2O | Syncephalastrum racemosum RR96 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
strains 45 and MNU82 | - |
| Aspergillus terreus | - |
- |
- |
| Brettanomyces naardenensis | - |
- |
- |
| Corynascus sepedonium | - |
- |
- |
| Debaryomyces sp. | - |
- |
- |
| Malbranchea cinnamomea | - |
- |
- |
| Nakazawaea holstii | - |
- |
- |
| Phaffia rhodozyma | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces sp. | - |
- |
- |
| Syncephalastrum racemosum | - |
- |
- |
| Syncephalastrum racemosum RR96 | - |
- |
- |
| Thermomyces lanuginosus | - |
- |
- |
| Trichosporon beigelii | - |
- |
- |
| Wickerhamomyces anomalus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Saccharomyces cerevisiae | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Aspergillus nidulans | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Nakazawaea holstii | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Phaffia rhodozyma | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Aspergillus terreus | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Saccharomyces sp. | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Wickerhamomyces anomalus | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Thermomyces lanuginosus | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Syncephalastrum racemosum | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Malbranchea cinnamomea | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Trichosporon beigelii | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Brettanomyces naardenensis | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Corynascus sepedonium | |
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | mode of action of the exo-amylase | Debaryomyces sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal cultivation conditions, overview | Saccharomyces cerevisiae | - |
| additional information | optimal cultivation conditions, overview | Aspergillus nidulans | - |
| additional information | optimal cultivation conditions, overview | Nakazawaea holstii | - |
| additional information | optimal cultivation conditions, overview | Phaffia rhodozyma | - |
| additional information | optimal cultivation conditions, overview | Aspergillus terreus | - |
| additional information | optimal cultivation conditions, overview | Saccharomyces sp. | - |
| additional information | optimal cultivation conditions, overview | Wickerhamomyces anomalus | - |
| additional information | optimal cultivation conditions, overview | Thermomyces lanuginosus | - |
| additional information | optimal cultivation conditions, overview | Syncephalastrum racemosum | - |
| additional information | optimal cultivation conditions, overview | Malbranchea cinnamomea | - |
| additional information | optimal cultivation conditions, overview | Trichosporon beigelii | - |
| additional information | optimal cultivation conditions, overview | Brettanomyces naardenensis | - |
| additional information | optimal cultivation conditions, overview | Corynascus sepedonium | - |
| additional information | optimal cultivation conditions, overview | Debaryomyces sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
| additional information | - |
- |
Phaffia rhodozyma |
| additional information | - |
- |
Aspergillus terreus |
| additional information | - |
- |
Thermomyces lanuginosus |
| additional information | - |
- |
Syncephalastrum racemosum |
| additional information | - |
- |
Malbranchea cinnamomea |
| additional information | - |
- |
Trichosporon beigelii |
| additional information | - |
- |
Brettanomyces naardenensis |
| additional information | - |
- |
Corynascus sepedonium |
| additional information | - |
strain 45 | Aspergillus nidulans |
| 0.45 | - |
- |
Wickerhamomyces anomalus |
| 0.475 | - |
- |
Nakazawaea holstii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| starch + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| starch + H2O | - |
Nakazawaea holstii | ? | - |
? | |
| starch + H2O | - |
Phaffia rhodozyma | ? | - |
? | |
| starch + H2O | - |
Aspergillus terreus | ? | - |
? | |
| starch + H2O | - |
Saccharomyces sp. | ? | - |
? | |
| starch + H2O | - |
Wickerhamomyces anomalus | ? | - |
? | |
| starch + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
| starch + H2O | - |
Syncephalastrum racemosum | ? | - |
? | |
| starch + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
| starch + H2O | - |
Trichosporon beigelii | ? | - |
? | |
| starch + H2O | - |
Brettanomyces naardenensis | ? | - |
? | |
| starch + H2O | - |
Corynascus sepedonium | ? | - |
? | |
| starch + H2O | - |
Debaryomyces sp. | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Saccharomyces cerevisiae | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Nakazawaea holstii | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Phaffia rhodozyma | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Aspergillus terreus | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Saccharomyces sp. | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Wickerhamomyces anomalus | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Thermomyces lanuginosus | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Syncephalastrum racemosum | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Malbranchea cinnamomea | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Trichosporon beigelii | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Brettanomyces naardenensis | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Corynascus sepedonium | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Debaryomyces sp. | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran, the enzyme from strain MNU82 utilizes raw and cooked starch | Aspergillus nidulans | ? | - |
? | |
| starch + H2O | - |
Syncephalastrum racemosum RR96 | ? | - |
? | |
| starch + H2O | starch substrate of different sources, e.g. wheat, wheat bran, rice bran | Syncephalastrum racemosum RR96 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Phaffia rhodozyma |
| 45 | - |
- |
Brettanomyces naardenensis |
| 60 | - |
- |
Aspergillus nidulans |
| 60 | - |
- |
Syncephalastrum racemosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Syncephalastrum racemosum |
| 5 | - |
- |
Brettanomyces naardenensis |
| 5.5 | 7 | enzyme from strain 45 | Aspergillus nidulans |
| 6 | - |
- |
Phaffia rhodozyma |
| 6.5 | - |
enzyme from strain MNU82 | Aspergillus nidulans |
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