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Literature summary for 3.2.1.2 extracted from
- Catalytic mechanism of beta-amylase from Bacillus cereus var. mycoides: chemical rescue of hydrolytic activity for a catalytic site mutant (Glu367-->Ala) by azide (2002), J. Biochem., 131, 587-591.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E172A | catalytic site mutant, no hydrolytic activity, no rescue of activity by 2 M azide | Bacillus cereus |
| E172A/E367A | catalytic site double mutant, no hydrolytic activity, no rescue of activity by 2 M azide | Bacillus cereus |
| E367A | catalytic site mutant, no hydrolytic activity in the absence of azide, in the presence of 2 M azide the mutant enzyme hydrolyzes maltopentaose at pH 7 and 25°C producing maltose, mechanism | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
var. mycoides | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| E172A, E367A and E172A/E367A mutant enzymes | Bacillus cereus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | catalytic mechanism, reaction mechanism | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltopentaose + H2O | beta-amylase is an inverting enzyme that hydrolyzes the alpha-1,4-glucosidic linkage of the substrate liberating beta-maltose from the non-reducing end, catalytic mechanism, Glu-172 acts as general acid, Glu-367 acts as general base | Bacillus cereus | ? | - |
? |  |
| starch + H2O | beta-amylase is an inverting enzyme that hydrolyzes the alpha-1,4-glucosidic linkage of the substrate liberating beta-maltose from the non-reducing end, catalytic mechanism, Glu-172 acts as general acid, Glu-367 acts as general base | Bacillus cereus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus cereus |
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