Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Geobacillus stearothermophilus | |
Al3+ | mild inhibitor | Geobacillus stearothermophilus | |
Cd2+ | - |
Geobacillus stearothermophilus | |
EDTA | non-competitive | Geobacillus stearothermophilus | |
Fe3+ | - |
Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
iodoacetic acid | - |
Geobacillus stearothermophilus | |
Mg2+ | mild inhibitor | Geobacillus stearothermophilus | |
Mn2+ | mild inhibitor | Geobacillus stearothermophilus | |
Ni2+ | - |
Geobacillus stearothermophilus | |
Pb2+ | - |
Geobacillus stearothermophilus | |
PCMB | - |
Geobacillus stearothermophilus | |
Zn2+ | mild inhibitor | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 1.25 mg/ml for starch | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | stimulates | Geobacillus stearothermophilus | |
Ca2+ | stimulates | Geobacillus stearothermophilus | |
K+ | stimulates | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | 85°C: 83% of maximal activity, 90°C: 32.8% of maximal activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | pH 5.5.: about 50% of maximal activity, pH 10.0: about 40% of maximal activity | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11.5 | 20°C, 24 h, stable. Sharp decrease in activity at pH 12.0 | Geobacillus stearothermophilus |