Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-cyclodextrin | - |
Thermoanaerobacterium thermosulfurigenes | |
beta-cyclodextrin | - |
Thermoanaerobacterium thermosulfurigenes | |
PCMB | - |
Thermoanaerobacterium thermosulfurigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 2.29 mg/ml for soluble starch at 60°C, 1.68 mg/ml for soluble starch at 75°C | Thermoanaerobacterium thermosulfurigenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermoanaerobacterium thermosulfurigenes | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Thermoanaerobacterium thermosulfurigenes |
210000 | - |
gel filtration | Thermoanaerobacterium thermosulfurigenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacterium thermosulfurigenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4215 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose + ? | - |
? | |
amylose + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose | - |
? | |
glycogen + H2O | - |
Thermoanaerobacterium thermosulfurigenes | beta-maltose | - |
? | |
additional information | no activity with pullulan | Thermoanaerobacterium thermosulfurigenes | ? | - |
? | |
starch + H2O | soluble | Thermoanaerobacterium thermosulfurigenes | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, SDS-PAGE | Thermoanaerobacterium thermosulfurigenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 55% of maximal activity, 80°C: about 65% of maximal activity | Thermoanaerobacterium thermosulfurigenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
stability is greatly enhanced by addition of 5 mM CaCl2 | Thermoanaerobacterium thermosulfurigenes |
70 | - |
2 h, stable in absence of substrate or Ca2+ | Thermoanaerobacterium thermosulfurigenes |
80 | - |
unstable in absence of substrate or Ca2+, stable in presence of 5 mM Ca2+ or 1% substrate | Thermoanaerobacterium thermosulfurigenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | pH 2.0: about 35% of maximal activity, pH 8.0: about 30% of maximal activity | Thermoanaerobacterium thermosulfurigenes |
5 | 6 | more than 80% of maximal activity at pH 5.0 and at pH 6.0 | Thermoanaerobacterium thermosulfurigenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | 70°C, in presence of 5 mM CaCl2 | Thermoanaerobacterium thermosulfurigenes |