Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tagged fusion protein | Triticum aestivum |
expressed in Escherichia coli as His-tagged fusion protein | Secale cereale |
Crystallization (Comment) | Organism |
---|---|
crystal structures of an inactive mutant of the wheat glucosidase complexed with the natural substrate DIMBOA-Glc, wheat and rye glucosidases complexed with an aglycone DIMBOA, and wheat and rye glucosidases complexed with an inhibitor 2-fluoro-2- deoxy-beta-D-glucose are analyzed | Triticum aestivum |
crystal structures of an inactive mutant of the wheat glucosidase complexed with the natural substrate DIMBOA-Glc, wheat and rye glucosidases complexed with an aglycone DIMBOA, and wheat and rye glucosidases complexed with an inhibitor 2-fluoro-2- deoxy-beta-D-glucose are analyzed | Secale cereale |
Protein Variants | Comment | Organism |
---|---|---|
E191A | crystals of the enzymatically inactive mutant E191A are soaked in a solution containing DIMBOA-Glc as substrate and 3.5M sodium formate as cryoprotectant, the structure is solved at a resolution of 2.2 A and the DIMBOA-Glc molecule is clearly defined. In the structure, the aglycone moiety is shown to be stabilized by interaction with the aromatic ring of W379 and by a hydrogen bond with T194 | Triticum aestivum |
E462A | inactive mutant, E462 plays a crucial role in hydrolysis of the substrate | Triticum aestivum |
G464S/S465L | mutation reduces the reaction efficiency toward DIBOA-Glc to 36% of wild type, whereas it enhances efficiency toward DIMBOA-Glc to 400% of wild type | Secale cereale |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-fluoro-2-deoxy-beta-D-glucose | - |
Secale cereale | |
2-fluoro-2-deoxy-beta-D-glucose | - |
Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | Q9FYS3 | - |
- |
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using HisTrap HP column and and Superdex 200 HR | Triticum aestivum |
using HisTrap HP column and and Superdex 200 HR | Secale cereale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-3-oxo-3,4,dihydro-2H-1,4,benzoxazin-2-yl beta-D-gluco-pyranoside + H2O | - |
Secale cereale | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
DIBOA-beta-D-glucoside + H2O | TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc | Triticum aestivum | DIBOA + beta-D-glucose | - |
? | |
DIBOA-glucoside + H2O | ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc | Secale cereale | DIBOA + beta-D-glucose | - |
? | |
DIMBOA-beta-D-glucoside + H2O | TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc | Triticum aestivum | DIMBOA + beta-D-glucose | - |
? | |
DIMBOA-glucoside + H2O | ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc | Secale cereale | DIMBOA + beta-D-glucose | - |
? | |
esculin + H2O | - |
Secale cereale | esculetin + beta-D-glucose | - |
? | |
genistin + H2O | - |
Secale cereale | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzoxazinone-glucoside beta-D-glucosidase | - |
Triticum aestivum |
benzoxazinone-glucoside beta-D-glucosidase | - |
Secale cereale |
ScGlu | - |
Secale cereale |
TaGlu1 | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Triticum aestivum |
30 | - |
assay at | Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Triticum aestivum |
5.5 | - |
assay at | Secale cereale |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
DIMBOA-glucoside | pH and temperature not specified in the publication | Secale cereale | |
150 | - |
DIBOA-glucoside | pH and temperature not specified in the publication | Secale cereale | |
150 | - |
DIBOA-beta-D-glucoside | pH and temperature not specified in the publication | Triticum aestivum | |
4100 | - |
DIMBOA-beta-D-glucoside | pH and temperature not specified in the publication | Triticum aestivum |