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Literature summary for 3.2.1.182 extracted from
- Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings (2000), Planta, 210, 432-438.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
esculin | pH 5.5, 30°C | Triticum aestivum |  |
| 0.27 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 0.67 | - |
4-nitrophenyl beta-D-fucoside | pH 5.5, 30°C | Triticum aestivum | |
| 1.34 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 1.7 | - |
4-nitrophenyl beta-D-glucoside | pH 5.5, 30°C | Triticum aestivum | |
| 1.78 | - |
4-nitrophenyl beta-D-galactoside | pH 5.5, 30°C | Triticum aestivum | |
| 2.02 | - |
7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin-2-yl beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.11 | - |
4-nitrophenyl beta-D-xyloside | pH 5.5, 30°C | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
enzyme is found to be present as oligomeric forms with a molecular mass of 260-300 kDa comprising 60- and 58-kDa monomers | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using gel filtration | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Triticum aestivum | - |
| seedling | isolated from | Triticum aestivum | - |
| shoot | - |
Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O | the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc | Triticum aestivum | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
| 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O | the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc | Triticum aestivum | 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-fucoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-fucose | - |
? | |
| 4-nitrophenyl beta-D-galactoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-xylose | - |
? | |
| 7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin-2-yl beta-D-glucopyranoside + H2O | - |
Triticum aestivum | 2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin + beta-D-glucose | - |
? | |
| esculin + H2O | - |
Triticum aestivum | esculetin + beta-D-glucose | - |
? | |
| salicin + H2O | - |
Triticum aestivum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Triticum aestivum |
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