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Literature summary for 3.2.1.18 extracted from
- A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola (2013), Mol. Microbiol., 89, 842-856.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Treponema denticola |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0187 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | in 70 mM sodium citrate, at pH 7.0 and 37°C | Treponema denticola |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Treponema denticola | 9986 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59800 | - |
x * 59800, calculated from amino acid sequence | Treponema denticola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Treponema denticola | Q73QH2 | - |
- |
| Treponema denticola ATCC 35405 | Q73QH2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Treponema denticola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Treponema denticola | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Treponema denticola ATCC 35405 | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59800, calculated from amino acid sequence | Treponema denticola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neuraminidase | - |
Treponema denticola |
| TDE0471 | - |
Treponema denticola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Treponema denticola |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme contributes to the virulence of Treponema denticola. The enzyme protects Treponema denticola from serum killing by preventing the deposition of membrane attack complexes on the bacterial cell surface | Treponema denticola |
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Release 2023.1 (January 2023)
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