Literature summary for 3.2.1.18 extracted from

Baek, Y.H.; Song, M.S.; Lee, E.Y.; Kim, Y.I.; Kim, E.H.; Park, S.J.; Park, K.J.; Kwon, H.I.; Pascua, P.N.; Lim, G.J.; Kim, S.; Yoon, S.W.; Kim, M.H.; Webby, R.J.; Choi, Y.K.
Profiling and characterization of potentially multidrug-resistant influenza neuraminidase 1 (N1) strains against neuraminidase inhibitors (2015), J. Virol., 89, 287-299.

Protein Variants

Protein Variants	Comment	Organism
D198N	the mutation moderately affects the susceptibility to neuraminidase inhibitors	influenza A virus
D198N/H274Y	the mutation confers reduced inhibition to zanamivir, oseltamivir, and peramivir	influenza A virus
D198N/R152K	inactive	influenza A virus
D198N/R292K	inactive	influenza A virus
E119A	the mutation confers reduced inhibition to zanamivir	influenza A virus
E119A/D198N	inactive	influenza A virus
E119A/H274Y	the mutation confers reduced inhibition to zanamivir, oseltamivir, and peramivir	influenza A virus
E119A/R152K	inactive	influenza A virus
E119A/R292K	inactive	influenza A virus
E119D	the mutation confers reduced inhibition to zanamivir, oseltamivir, and peramivir	influenza A virus
E119D/D198N	inactive	influenza A virus
E119D/H274Y	the mutation confers reduced inhibition to zanamivir, oseltamivir, and peramivir	influenza A virus
E119D/R152K	inactive	influenza A virus
E119D/R292K	inactive	influenza A virus
E119G	the mutation confers reduced inhibition to zanamivir	influenza A virus
E119G/D198N	inactive	influenza A virus
E119G/H274Y	the mutation confers reduced inhibition to zanamivir, oseltamivir, and peramivir	influenza A virus
E119G/R152K	inactive	influenza A virus
E119G/R292K	inactive	influenza A virus
H274Y	the mutation confers highly reduced inhibition to oseltamivir (1131.4fold increase in IC50 values compared to the wild type) and reduced inhibition to peramivir by 50.6fold	influenza A virus
N294S	the mutation confers reduced inhibition to oseltamivir (63.6fold increase in IC50 compared to the wild type)	influenza A virus
N294S/H274Y	inactive	influenza A virus
R152K	the mutant exhibits reduced inhibition to oseltamivir (17.9fold in IC50 values) but shows wild type inhibition to zanamivir and peramivir	influenza A virus
R152K/H274Y	inactive	influenza A virus
R292K	the mutant exhibits reduced inhibition to oseltamivir (33fold in IC50 values) but shows wild type inhibition to zanamivir and peramivir	influenza A virus
R292K/H274Y	inactive	influenza A virus

Inhibitors

Inhibitors	Comment	Organism
oseltamivir carboxylate	-	influenza A virus
peramivir	-	influenza A virus
zanamivir	-	influenza A virus

Organism

Organism	UniProt	Comment	Textmining
influenza A virus	A9YLL2	-	-
influenza A virus	C3W5S3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O	-	influenza A virus	4-methylumbelliferone + N-acetylneuraminic acid	-	?

Synonyms

Synonyms	Comment	Organism
neuraminidase 1	-	influenza A virus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.0000003	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000003	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000006	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000007	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000007	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000007	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000009	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000009	-	mutant enzyme D198N, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000011	-	mutant enzyme R152K, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000011	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000013	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000014	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000014	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000014	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000016	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000019	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000021	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000022	-	wild type enzyme, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000022	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000029	-	mutant enzyme D198N, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000029	-	mutant enzyme D198N/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000031	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000037	-	mutant enzyme D198N, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.000004	-	mutant enzyme R152K, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000054	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000068	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000095	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	peramivir
0.00001019	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000125	-	mutant enzyme ER152K, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000136	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000152	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir
0.000016	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000218	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000231	-	mutant enzyme R292K, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000244	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000245	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000304	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir
0.000031	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000445	-	mutant enzyme N294S, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0000499	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000508	-	mutant enzyme D198N/H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0000521	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000957	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000959	-	mutant enzyme E119A, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0000975	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0001221	-	mutant enzyme E119D/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0001233	-	mutant enzyme E119D/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0001421	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	peramivir
0.0001447	-	mutant enzyme E119G/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0001572	-	mutant enzyme E119G/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0001916	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0002019	-	mutant enzyme E119G/H274Y, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0002511	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.0003514	-	mutant enzyme E119D/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.000525	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.00059	-	mutant enzyme D198N/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.000792	-	mutant enzyme H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.00082	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.0008329	-	mutant enzyme E119G, pH and temperature not specified in the publication	influenza A virus	zanamivir
0.001762	-	mutant enzyme E119G/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.001867	-	mutant enzyme E119D, pH and temperature not specified in the publication	influenza A virus	peramivir
0.002117	-	mutant enzyme E119D/H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir
0.002682	-	mutant enzyme E119D/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.003367	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	oseltamivir carboxylate
0.003492	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir
0.004374	-	mutant enzyme E119A/H274Y, pH and temperature not specified in the publication	influenza A virus	peramivir