Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacteroides thetaiotaomicron |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 4000, 0.2 M ammonium citrate tri-basic (pH 7.0) and 0.1 M Tris pH 8.5 | Bacteroides thetaiotaomicron |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2,3-dehydro-N-acetyl neuraminic acid | competitive inhibition | Bacteroides thetaiotaomicron | |
Cu2+ | 2.9% residual activity at 5 mM | Bacteroides thetaiotaomicron | |
Fe2+ | 33.7% residual activity at 5 mM | Bacteroides thetaiotaomicron | |
additional information | EDTA does not cause a significant decrease in activity | Bacteroides thetaiotaomicron | |
SDS | 0.9% residual activity at 5 mM | Bacteroides thetaiotaomicron | |
Zn2+ | 31.9% residual activity at 5 mM | Bacteroides thetaiotaomicron |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
0.16 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
0.29 | - |
colominic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
0.49 | - |
sialyl-alpha-2,6-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
1.47 | - |
sialyl-alpha-2,3-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the activity is maintained more than 94% in the presence of Ca2+ at concentrations up to 5 mM | Bacteroides thetaiotaomicron | |
Mg2+ | the activity is maintained more than 96% in the presence of Mg2+ at concentrations up to 5 mM | Bacteroides thetaiotaomicron | |
Mn2+ | the activity is maintained more than 69% in the presence of Mn2+ at concentrations up to 5 mM | Bacteroides thetaiotaomicron | |
Ni2+ | the activity is maintained more than 75% in the presence of Ni2+ at concentrations up to 5 mM | Bacteroides thetaiotaomicron |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q8AAK9 | - |
- |
Bacteroides thetaiotaomicron VPI-5482 | Q8AAK9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Bacteroides thetaiotaomicron |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.6 | - |
with transferrin as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
18.2 | - |
with alpha1-acid glycoprotein as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
35 | - |
with fetuin as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
64.1 | - |
with colominic acid as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
97.7 | - |
with sialyl-alpha-2,3-lactose as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
100.9 | - |
with sialyl-alpha-2,6-lactose as substrate, pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O | - |
Bacteroides thetaiotaomicron | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O | - |
Bacteroides thetaiotaomicron | 4-nitrophenol + N-acetylneuraminic acid | - |
? | |
alpha1-acid glycoprotein + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
alpha1-acid glycoprotein + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
colominic acid + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
colominic acid + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
fetuin + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
fetuin + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
sialyl-alpha-2,3-lactose + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
sialyl-alpha-2,3-lactose + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
sialyl-alpha-2,6-lactose + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
sialyl-alpha-2,6-lactose + H2O | - |
Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
transferrin + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuraminidase | - |
Bacteroides thetaiotaomicron |
sialidase | - |
Bacteroides thetaiotaomicron |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacteroides thetaiotaomicron |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 60% of activity is maintained from 30 to 50°C | Bacteroides thetaiotaomicron |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82.4 | - |
4-nitrophenyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
146.7 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
157.6 | - |
colominic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
161 | - |
sialyl-alpha-2,6-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
313.5 | - |
sialyl-alpha-2,3-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in 50 mM Tris-HCl buffer | Bacteroides thetaiotaomicron |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | more than 70% of activity is maintained from pH 6.0 to 8.0 | Bacteroides thetaiotaomicron |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
2-deoxy-2,3-dehydro-N-acetyl neuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
213.3 | - |
sialyl-alpha-2,3-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
328.6 | - |
sialyl-alpha-2,6-lactose | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
541.2 | - |
colominic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
729.2 | - |
4-nitrophenyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron | |
916.9 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | pH 7.0 and 37°C | Bacteroides thetaiotaomicron |