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Literature summary for 3.2.1.18 extracted from

  • Yeung, J.H.; Telford, J.C.; Shidmoossavee, F.S.; Bennet, A.J.; Taylor, G.L.; Moore, M.M.
    Kinetic and structural evaluation of selected active site mutants of the Aspergillus fumigatus KDNase (sialidase) (2013), Biochemistry, 52, 9177-9186.
    View publication on PubMed
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
D84A the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase Aspergillus fumigatus
R171L the mutant has higher activity on a N-acetylneuraminic acid-based substrate than wild type enzyme Aspergillus fumigatus
Y358H the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WQS0
-
-
Aspergillus fumigatus Af293 Q4WQS0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S-100H gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O preferred substrate Aspergillus fumigatus ?
-
 ?
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O preferred substrate Aspergillus fumigatus Af293 ?
-
 ?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Aspergillus fumigatus ?
-
 ?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Aspergillus fumigatus Af293 ?
-
 ?
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
-
 Aspergillus fumigatus 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
 ?
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
-
 Aspergillus fumigatus Af293 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
 ?

Synonyms

Synonyms Comment Organism
KDNase
-
 Aspergillus fumigatus
sialidase
-
 Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
200
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
330
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
1620
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
8900
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
11600
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
14500
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
19000
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
26800
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
47700
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
53500
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
74200
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus