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Literature summary for 3.2.1.18 extracted from

  • Sandbhor, M.S.; Soya, N.; Albohy, A.; Zheng, R.B.; Cartmell, J.; Bundle, D.R.; Klassen, J.S.; Cairo, C.W.
    Substrate recognition of the membrane-associated sialidase NEU3 requires a hydrophobic aglycone (2011), Biochemistry, 50, 6753-6762.
    View publication on PubMed
Search for more literature for 3.2.1.18

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme NEU3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Homo sapiens 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
ganglioside GM3 + H2O
-
 Homo sapiens ?
-
 ?
additional information substrate recognition of the membrane-associated sialidase NEU3 requires a hydrophobic aglycone, two-site model for enzyme recognition, requiring interaction at both the Neu5Ac residue and the hydrophobic aglycone. Evaluation of series of 11 synthetic trisaccharides. NEU3 substrate activity is directly dependent upon the hydrophobicity of the aglycone but has no apparent requirement for features of the ceramide headgroup. Trisaccharides with incorporated azide groups in the Neu5Ac residue at either C9 or the N5-Ac position are substrates, and in the case of the N5-azidoacetyl derivative, the activity is superior to that of GM3. Incorporation of larger aryl groups is tolerated only at C9, but not at N5-Ac Homo sapiens ?
-
 ?
Neu5(4-[(2,4-dinitrophenoxy)methyl]-1H-1,2,3-triazol-1-yl)Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5(azido)Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-(CH2)8COOCH3 + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-CH2(CH3)CH2CH2CH3 + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-CH2Ph + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-butyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-N3 + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac9(2,4-dinitrobenzoyl)N-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac9N-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?
Neu5Ac9N3-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Neu3
-
 Homo sapiens
sialidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 assay at Homo sapiens