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Literature summary for 3.2.1.18 extracted from

  • Yongkiettrakul, S.; Boonyapakron, K.; Jongkaewwattana, A.; Wanitchang, A.; Leartsakulpanich, U.; Chitnumsub, P.; Eurwilaichitr, L.; Yuthavong, Y.
    Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain (2009), J. Virol. Methods, 156, 44-51.
    View publication on PubMed
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
fusion of gene encoding the neuraminidase N1 head domain residues 63-449 with the Saccharomyces cerevisiae alpha-factor secretion signal and expression in Pichia pastoris unidentified influenza virus

Protein Variants

Protein Variants Comment Organism
additional information fusion of gene encoding the neuraminidase N1 head domain residues 63-449 with the Saccharomyces cerevisiae alpha-factor secretion signal and expression in Pichia pastoris. Recombinant product is a soluble protein with similar kinetic characteristics as wild-type unidentified influenza virus

Inhibitors

Inhibitors Comment Organism Structure
oseltamivir carboxylate
-
 unidentified influenza virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid wild-type, pH 6.5, 37°C unidentified influenza virus
0.018
-
 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid recombinant head domain, pH 6.5, 37°C unidentified influenza virus

Organism

Organism UniProt Comment Textmining
unidentified influenza virus
-
 A/Viet Nam/DT-036/2005 type H5N1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein treatment of recombinant N1 head domain with glycopeptidase F generates a 45000 Da product unidentified influenza virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
314
-
 recombinant head domain, pH 6.5, 37°C unidentified influenza virus
378
-
 wild-type, pH 6.5, 37°C unidentified influenza virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
-
 unidentified influenza virus 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000013
-
 oseltamivir carboxylate wild-type, pH 6.5, 37°C unidentified influenza virus
0.0000024
-
 oseltamivir carboxylate recombinant head domain, pH 6.5, 37°C unidentified influenza virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000121
-
 wild-type, pH 6.5, 37°C unidentified influenza virus oseltamivir carboxylate
0.0000182
-
 recombinant head domain, pH 6.5, 37°C unidentified influenza virus oseltamivir carboxylate