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Literature summary for 3.2.1.18 extracted from

  • Tringali, C.; Papini, N.; Fusi, P.; Croci, G.; Borsani, G.; Preti, A.; Tortora, P.; Tettamanti, G.; Venerando, B.; Monti, E.
    Properties of recombinant human cytosolic sialidase HsNEU2: The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules (2004), J. Biol. Chem., 279, 3169-3179.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
octyl glucoside highest activation (1.3-2.2fold) at 0.08% w/v concentration Homo sapiens
sodium deoxycholate highest activation (1.1-1.8.5fold) at 0.03% w/v concentration Homo sapiens
Triton X-100 highest activation (1.5-2.5fold) at 0.02% w/v concentration Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

General Stability

General Stability Organism
the enzyme is unstable upon freezing at -20°C, 50% loss of activity after one cycle of freezing-thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ above 1 mM Homo sapiens
Cu2+ above 0.01 mM Homo sapiens
K+ above 1 mM Homo sapiens
Na+ above 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
ganglioside GD1a 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
0.24
-
ganglioside GM3 37°C, pH 5.6 or 7.0, activated by 0.02% Triton X-100 Homo sapiens
0.28
-
alpha(2-3)sialylparagloboside 37°C, pH 5.6 or 7.0 Homo sapiens
0.31
-
alpha(2-3)sialyllactose 37°C, pH 5.6 or 7.0 Homo sapiens
0.38
-
ganglioside GT1b 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
0.4
-
ganglioside GM3 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
0.43
-
alpha(2-3)sialylparagloboside 37°C, pH 5.6 or 7.0 Homo sapiens
0.44
-
4-methylumbelliferyl alpha-NeuAc 37°C, pH 5.6 or 7.0 Homo sapiens
0.51
-
ganglioside GD1b 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
0.59
-
ganglioside GD1b 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
0.62
-
ganglioside GT1b 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
0.91
-
ganglioside GD1a 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ slight activation up to 0.01 mM Homo sapiens
Na+ slight activation up to 0.01 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, stable for 1 year Homo sapiens
4°C, 50% loss of activity after 24 h Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-NeuAc + H2O
-
Homo sapiens 4-methylumbelliferone + alpha-neuAc
-
?
alpha(2-3)sialyllactose + H2O
-
Homo sapiens sialic acid + lactose
-
?
alpha(2-3)sialylparagloboside + H2O
-
Homo sapiens ?
-
?
alpha-acid glycoprotein + H2O
-
Homo sapiens ?
-
?
bovine gland submaxillary gland mucin + H2O
-
Homo sapiens ?
-
?
fetuin + H2O
-
Homo sapiens ?
-
?
ganglioside GD1a + H2O
-
Homo sapiens ?
-
?
ganglioside GD1b + H2O
-
Homo sapiens ?
-
?
ganglioside GM3 + H2O
-
Homo sapiens ?
-
?
ganglioside GT1b + H2O
-
Homo sapiens ?
-
?
transferrin + H2O
-
Homo sapiens ?
-
?
vesicular GM3 + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
HsNEU2
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
24 h, 50% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Homo sapiens