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Literature summary for 3.2.1.18 extracted from
- Characterization of sialidase from bloodstream forms of Trypanosoma vivax (2006), Cell Biochem. Funct., 24, 71-77.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | activates | Trypanosoma vivax |  |
| dithiothreitol | 1 mM, activates 2fold | Trypanosoma vivax | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid | competitive | Trypanosoma vivax | |
| 2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid | - |
Trypanosoma vivax | |
| N-bromosuccinimide | - |
Trypanosoma vivax | |
| p-hydroxy oxamic acid | - |
Trypanosoma vivax | |
| p-hydroxymercuribenzoate | 0.001 mM, complete inhibition | Trypanosoma vivax | |
| p-hydroxyoxamic acid | competitive | Trypanosoma vivax | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
Neu5Ac(alpha2,3)-lactose | pH 6.5, 37°C | Trypanosoma vivax | |
| 0.17 | - |
Neu5Ac(2-3)-alpha-lactose | 37°C, pH 6.7 | Trypanosoma vivax | |
| 0.45 | - |
fetuin | pH 6.5, 37°C | Trypanosoma vivax | |
| 0.45 | - |
fetuin | 37°C, pH 6.7 | Trypanosoma vivax |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | complete inhibition at 1 mM | Trypanosoma vivax | |
| Hg2+ | complete inhibition at 1 mM | Trypanosoma vivax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Trypanosoma vivax |
| 68000 | - |
gel filtration | Trypanosoma vivax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma vivax | - |
Agai strain | - |
| Trypanosoma vivax | - |
Agari strain | - |
| Trypanosoma vivax Agai | - |
Agai strain | - |
| Trypanosoma vivax Agari | - |
Agari strain | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trypanosoma vivax |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma vivax |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bloodstream form | - |
Trypanosoma vivax | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31 | - |
- |
Trypanosoma vivax |
| 31.02 | - |
- |
Trypanosoma vivax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fetuin + H2O | - |
Trypanosoma vivax | ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma vivax Agai | ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma vivax Agari | ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma vivax | Neu5Ac + ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma vivax Agai | Neu5Ac + ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma vivax Agari | Neu5Ac + ? | - |
? | |
| additional information | the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI | Trypanosoma vivax | ? | - |
? | |
| additional information | the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI | Trypanosoma vivax Agai | ? | - |
? | |
| additional information | the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI | Trypanosoma vivax Agari | ? | - |
? | |
| Neu5Ac(2-3)-alpha-lactose + H2O | - |
Trypanosoma vivax | Neu5Ac + lactose | - |
? | |
| Neu5Ac(2-3)-alpha-lactose + H2O | - |
Trypanosoma vivax Agai | Neu5Ac + lactose | - |
? | |
| Neu5Ac(2-3)-alpha-lactose + H2O | - |
Trypanosoma vivax Agari | Neu5Ac + lactose | - |
? | |
| Neu5Ac(2-6)-alpha-lactose + H2O | - |
Trypanosoma vivax | Neu5Ac + lactose | - |
? | |
| Neu5Ac(2-6)-alpha-lactose + H2O | - |
Trypanosoma vivax Agai | Neu5Ac + lactose | - |
? | |
| Neu5Ac(2-6)-alpha-lactose + H2O | - |
Trypanosoma vivax Agari | Neu5Ac + lactose | - |
? | |
| Neu5Ac(alpha2,3)lactose + H2O | - |
Trypanosoma vivax | Neu5Ac + lactose | - |
? | |
| Neu5Ac(alpha2,6)-lactose + H2O | - |
Trypanosoma vivax | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Trypanosoma vivax |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Trypanosoma vivax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Trypanosoma vivax |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | complete cessation of activity at pH 4 and 8.5 | Trypanosoma vivax |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid | - |
Trypanosoma vivax | |
| 0.06 | - |
(6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid | pH 6.5, 37°C | Trypanosoma vivax | |
| 0.15 | - |
p-hydroxy oxamic acid | - |
Trypanosoma vivax | |
| 0.15 | - |
p-hydroxyoxamic acid | pH 6.5, 37°C | Trypanosoma vivax | |
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