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Literature summary for 3.2.1.18 extracted from

  • Nok, A.J.; Nzelibe, H.C.; Yako, S.K.
    Trypanosoma evansi sialidase: surface localization, properties and hydrolysis of ghost red blood cells and brain cells-implications in trypanosomiasis (2003), Z. Naturforsch. C, 58, 594-601.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid i.e. NeuAc2,3en, competitive Trypanosoma evansi
Cu2+
-
Trypanosoma evansi
N-bromosuccinimide competitive Trypanosoma evansi
N-ethylmaleimide competitive Trypanosoma evansi
p-chloromercuribenzoate 50% inhibition at 0.065 mM Trypanosoma evansi
Zn2+
-
Trypanosoma evansi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid pH 6.5, 37°C Trypanosoma evansi
0.0029
-
fetuin pH 5.5, 37°C Trypanosoma evansi
0.0049
-
alpha(2-3)-sialyllactose pH 6.5, 37°C Trypanosoma evansi

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Trypanosoma evansi 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Trypanosoma evansi

Organism

Organism UniProt Comment Textmining
Trypanosoma evansi
-
isolated from blood of infected camels, STIB 731-AA, IL 1392
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Trypanosoma evansi

Purification (Commentary)

Purification (Comment) Organism
310fold by solubilization with Triton CF 54 and ion exchange and fetuin-affinity chromatographic steps Trypanosoma evansi

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
174
-
purified enzyme Trypanosoma evansi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
Trypanosoma evansi 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
?
alpha(2-3)-sialyllactose + H2O
-
Trypanosoma evansi sialic acid + lactose
-
?
alpha(2-6)-sialyllactose + H2O
-
Trypanosoma evansi sialic acid + lactose
-
?
colomic acid + H2O low activity Trypanosoma evansi sialic acid + lactose
-
?
fetuin + H2O
-
Trypanosoma evansi sialic acid + ?
-
?
ganglioside GM2 + H2O
-
Trypanosoma evansi sialic acid + ?
-
?
mucin + H2O
-
Trypanosoma evansi sialic acid + ?
-
?
Neu5,9-diAc(2-3)-alpha-lactose + H2O low activity Trypanosoma evansi ?
-
?
red blood ghost cells + H2O desialylation, preference for different species: in descending order mouse, rat, camel, goat, and dog Trypanosoma evansi ?
-
?
transferrin + H2O
-
Trypanosoma evansi sialic acid + ?
-
?

Synonyms

Synonyms Comment Organism
sialidase
-
Trypanosoma evansi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Trypanosoma evansi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Trypanosoma evansi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0053
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
0.04
-
p-chloromercuribenzoate pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
0.042
-
N-bromosuccinimide pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
0.053
-
N-ethylmaleimide pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi