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Literature summary for 3.2.1.17 extracted from

  • Guo, Y.; He, H.
    Identification and characterization of a goose-type lysozyme from sewage snail Physa acuta (2014), Fish Shellfish Immunol., 39, 321-325.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genomic library construction, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovrisu transfection system via host Escherichia coli strain coli DH10Bac Physella acuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
x * 21000, SDS-PAGE, x * 21200, about, sequence calculation Physella acuta
21200
-
x * 21000, SDS-PAGE, x * 21200, about, sequence calculation Physella acuta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Physella acuta the recombinant enzyme displays the lytic activity of g-type lysozyme with other organisms against Micrococcus lysodikicus ?
-
?

Organism

Organism UniProt Comment Textmining
Physella acuta G8CYM6 collected from Xiaoyue River, Chaoyang district, Beijing
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas high enzyme expression level Physella acuta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme displays the lytic activity of g-type lysozyme with other organisms against Micrococcus lysodikicus Physella acuta ?
-
?

Subunits

Subunits Comment Organism
? x * 21000, SDS-PAGE, x * 21200, about, sequence calculation Physella acuta

Synonyms

Synonyms Comment Organism
Goose-type lysozyme
-
Physella acuta
PALysG
-
Physella acuta

pI Value

Organism Comment pI Value Maximum pI Value
Physella acuta sequence calculation
-
6.38

General Information

General Information Comment Organism
evolution the conservative structure domains share high homology with other molluscan g-type lysozymes including the SLT domain, the substrate binding sites, the catalytic residues, three alpha-helices structures and six molluscan specific cysteines Physella acuta