Cloned (Comment) | Organism |
---|---|
genomic library construction, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovrisu transfection system via host Escherichia coli strain coli DH10Bac | Physella acuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, SDS-PAGE, x * 21200, about, sequence calculation | Physella acuta |
21200 | - |
x * 21000, SDS-PAGE, x * 21200, about, sequence calculation | Physella acuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Physella acuta | the recombinant enzyme displays the lytic activity of g-type lysozyme with other organisms against Micrococcus lysodikicus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physella acuta | G8CYM6 | collected from Xiaoyue River, Chaoyang district, Beijing | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | high enzyme expression level | Physella acuta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme displays the lytic activity of g-type lysozyme with other organisms against Micrococcus lysodikicus | Physella acuta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, SDS-PAGE, x * 21200, about, sequence calculation | Physella acuta |
Synonyms | Comment | Organism |
---|---|---|
Goose-type lysozyme | - |
Physella acuta |
PALysG | - |
Physella acuta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Physella acuta | sequence calculation | - |
6.38 |
General Information | Comment | Organism |
---|---|---|
evolution | the conservative structure domains share high homology with other molluscan g-type lysozymes including the SLT domain, the substrate binding sites, the catalytic residues, three alpha-helices structures and six molluscan specific cysteines | Physella acuta |