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Literature summary for 3.2.1.17 extracted from

  • Myrnes, B.; Seppola, M.; Johansen, A.; Overb?, K.; Callewaert, L.; Vanderkelen, L.; Michiels, C.W.; Nilsen, I.W.
    Enzyme characterisation and gene expression profiling of Atlantic salmon chicken- and goose-type lysozymes (2013), Dev. Comp. Immunol., 40, 11-19.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
c-type inhibitor Ivy complete inhibition Salmo salar
Ca2+ 34% activation at 5 mM, 150% activation at 10 mM, 280% at 5 mM of recombinant enzyme. 58% Inhibition at 10 mM, 97% at 50 mM of native enzyme, 66% inhibition at 20 mM, 97% at 50 mM of recombinant enzyme; 87% inhibition at 5 mM, 93% at 10 mM Salmo salar
g-type inhibitor PliG complete inhibition Salmo salar
additional information no inhibition by c-type inhibitor Ivy; no inhibition by g-type inhibitor PliG Salmo salar
Na+ 14% inhibition at 10 mM, 81% at 100 mM; activates the native enzyme 3.43fold at 50 mM, the recombinant enzyme 4.3fold at 100 mM, inhibition of native, not recombinant, enzyme at 200 mM Salmo salar

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 34% activation at 5 mM, 150% activation at 10 mM, 280% at 5 mM of recombinant enzyme. 58% Inhibition at 10 mM, 97% at 50 mM of native enzyme, 66% inhibition at 20 mM, 97% at 50 mM of recombinant enzyme Salmo salar
Na+ activates the native enzyme 3.43fold at 50 mM, the recombinant enzyme 4.3fold at 100 mM, inhibition of native, not recombinant, enzyme at 200 mM Salmo salar

Organism

Organism UniProt Comment Textmining
Salmo salar A6PZ97
-
-
Salmo salar B5XA65
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from head kidneys by ultrafiltration, cation exchange chromatography, ultrafiltration, and gel filtration Salmo salar

Source Tissue

Source Tissue Comment Organism Textmining
blood contains exclusively g-type lysozyme Salmo salar
-
gill contains exclusively g-type lysozyme Salmo salar
-
head kidney contains c-type and g-type lysozymes Salmo salar
-
liver contains exclusively g-type lysozyme Salmo salar
-
macrophage contains c-type and g-type lysozymes Salmo salar
-
additional information c-type lysozyme occurs only in head kidney and spleen Salmo salar
-
spleen contains c-type and g-type lysozymes Salmo salar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12500
-
purified enzyme, pH 6.0, temperature not specified in the publication Salmo salar
18800
-
purified enzyme, pH 6.0, temperature not specified in the publication Salmo salar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lyophilized cell wall of Micrococcus luteus + H2O
-
Salmo salar ?
-
?

Synonyms

Synonyms Comment Organism
c-type lysozyme
-
Salmo salar
chicken-type lysozyme
-
Salmo salar
g-type lysozyme
-
Salmo salar
Goose-type lysozyme
-
Salmo salar
n-SalC
-
Salmo salar
n-SalG
-
Salmo salar

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 70 n-SalC lysozyme displays a sigmoidally increasing activity profile from 4°C to 70°C Salmo salar
22 60 native g-type lysozyme activity slowly increases from a relatively high lytic activity at 4°C (45% of maximum) to peak lytic activity at 60°C. The recombinant enzyme reaches maximum activity at 22°C, high catalytic activity at 4°C and no activity at 60°C Salmo salar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 6 assay at Salmo salar

Expression

Organism Comment Expression
Salmo salar real-time PCR analysis reveals that in macrophages g-type lysozyme shows a constitutive gene expression level independent of lipopolysaccharides additional information
Salmo salar real-time PCR analysis reveals that the c-type lysozyme transcription in macrophages is highly inducible by lipopolysaccharide up

General Information

General Information Comment Organism
physiological function lysozymes represent important innate immune components against bacteria Salmo salar