Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis | Meretrix lusoria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no increase in activity at high ionic strength | Meretrix lusoria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13363 | - |
1 * 14000, lysozymes A and B, SDS-PAGE, 1 * 13382, lysozyme A, sequence calculation 1 * 13376, lysozyme A, mass spectrometry, 1 * 13363, lysozyme B, mass spectrometry | Meretrix lusoria |
13376 | - |
1 * 14000, lysozymes A and B, SDS-PAGE, 1 * 13382, lysozyme A, sequence calculation 1 * 13376, lysozyme A, mass spectrometry, 1 * 13363, lysozyme B, mass spectrometry | Meretrix lusoria |
13382 | - |
1 * 14000, lysozymes A and B, SDS-PAGE, 1 * 13382, lysozyme A, sequence calculation 1 * 13376, lysozyme A, mass spectrometry, 1 * 13363, lysozyme B, mass spectrometry | Meretrix lusoria |
14000 | - |
1 * 14000, lysozymes A and B, SDS-PAGE, 1 * 13382, lysozyme A, sequence calculation 1 * 13376, lysozyme A, mass spectrometry, 1 * 13363, lysozyme B, mass spectrometry | Meretrix lusoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meretrix lusoria | P86383 | i-type lysozyme, two isozymes lysozyme A and lysozyme B | - |
Purification (Comment) | Organism |
---|---|
native isozymes to homogeneity by ammonium sulfate fractionation, two steps of cation exchange chromatography, and isozyme separation by reversed phase gel filtration | Meretrix lusoria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
620 | - |
purified lysozyme A, pH 7.0, 25°C | Meretrix lusoria |
1227 | - |
purified lysozyme B, pH 7.0, 25°C | Meretrix lusoria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitohexaose + H2O | - |
Meretrix lusoria | ? | - |
? | |
chitopentaose + H2O | - |
Meretrix lusoria | ? | - |
? | |
lyophilized cell wall of Micrococcus luteus + H2O | - |
Meretrix lusoria | ? | - |
? | |
additional information | the enzyme shows also chitinase activity on glycol chitin as substrate, but no transglycosylation activity, and a higher number of subsites compared to hen egg-white enzyme | Meretrix lusoria | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14000, lysozymes A and B, SDS-PAGE, 1 * 13382, lysozyme A, sequence calculation 1 * 13376, lysozyme A, mass spectrometry, 1 * 13363, lysozyme B, mass spectrometry | Meretrix lusoria |
More | the enzyme shows no dimerization like the enzyme of bivalve Venerupis philippinarum due to the lack of residue Lys108 responsible of dimerization in the other organism | Meretrix lusoria |
Synonyms | Comment | Organism |
---|---|---|
i-type lysozyme | - |
Meretrix lusoria |
lysozyme A | - |
Meretrix lusoria |
lysozyme B | - |
Meretrix lusoria |
MLL-A | - |
Meretrix lusoria |
MLL-B | - |
Meretrix lusoria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, lytic activity, substrate lyophilized cell wall of Micrococcus luteus | Meretrix lusoria |
50 | - |
assay at, substrate N-acetylglucosamine oligomers | Meretrix lusoria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at, substrate N-acetylglucosamine oligomers | Meretrix lusoria |
6.5 | - |
substrate lyophilized cell wall of Micrococcus luteus, isozyme lysozyme A | Meretrix lusoria |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function relationhip of two isozymes of the invertebrate i-type lysozyme, active site residues are Glu18 and Asp30, substrate interaction via residues P44, Y45, Y47, H94, and p98, overview | Meretrix lusoria |