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Literature summary for 3.2.1.17 extracted from

  • Sziegat, F.; Silvers, R.; Haehnke, M.; Jensen, M.R.; Blackledge, M.; Wirmer-Bartoschek, J.; Schwalbe, H.
    Disentangling the coil: modulation of conformational and dynamic properties by site-directed mutation in the non-native state of hen egg white lysozyme (2012), Biochemistry, 51, 3361-3372.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzyme mutants in Escherichia coli strain BL21(DE3) in inclusion bodies Gallus gallus

Protein Variants

Protein Variants Comment Organism
additional information site-directed mutagenesis to modulate conformation and dynamic properties of the enzyme in non-native unfolded state, random-coil structure, modelling, and comprehensive analysis of non-native states of wild-type and mutant forms of the model protein lysozyme by nuclear magnetic resonance spectroscopy Gallus gallus
W108GA site-directed mutagenesis Gallus gallus
W111GA site-directed mutagenesis Gallus gallus
W123G site-directed mutagenesis Gallus gallus
W28GA site-directed mutagenesis Gallus gallus
W62GA site-directed mutagenesis Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
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-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant mutants in unfolded non-native state, solubilization of the recombinant mutants from Escherichia coli strain BL21(DE3) inclusion bodies in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, and 8 M urea, pH 7.5, centrifugation, and cation exchange chromatography with elution by 50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, and 4 M urea, pH 7.5 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Gallus gallus
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egg white
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Gallus gallus
-