Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression in Spodoptera frugiperda cells via baculovirus transfection method | Hirudo medicinalis |
recombinant enzyme expression in Spodoptera frugiperda cells via baculovirus transfection method | Hirudo verbana |
recombinant enzyme expression in Spodoptera frugiperda cells via baculovirus transfection method | Hirudo orientalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Hirudo medicinalis | - |
- |
extracellular | the enzyme is secreted | Hirudo verbana | - |
- |
extracellular | the enzyme is secreted | Hirudo orientalis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hirudo medicinalis | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | ? | - |
? | |
additional information | Hirudo verbana | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | ? | - |
? | |
additional information | Hirudo orientalis | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hirudo medicinalis | - |
- |
- |
Hirudo orientalis | - |
- |
- |
Hirudo verbana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | - |
Hirudo medicinalis | - |
salivary gland | - |
Hirudo verbana | - |
salivary gland | - |
Hirudo orientalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O | a synthetic fluorogenic substrate | Hirudo medicinalis | 4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose | - |
? | |
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O | a synthetic fluorogenic substrate | Hirudo verbana | 4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose | - |
? | |
4-methylumbelliferyl tetra N-acetyl-beta-chitotetraoside + H2O | a synthetic fluorogenic substrate | Hirudo orientalis | 4-methylumbelliferol + tetra N-acetyl-beta-chitotetraose | - |
? | |
chitohexaose + H2O | - |
Hirudo medicinalis | chitobiose + chitotetraose | mass spectrometry analysis | ? | |
chitohexaose + H2O | - |
Hirudo verbana | chitobiose + chitotetraose | mass spectrometry analysis | ? | |
chitohexaose + H2O | - |
Hirudo orientalis | chitobiose + chitotetraose | mass spectrometry analysis | ? | |
Micrococcus lysodeikticus cell wall + H2O | - |
Hirudo medicinalis | ? | - |
? | |
Micrococcus lysodeikticus cell wall + H2O | - |
Hirudo verbana | ? | - |
? | |
Micrococcus lysodeikticus cell wall + H2O | - |
Hirudo orientalis | ? | - |
? | |
additional information | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | Hirudo medicinalis | ? | - |
? | |
additional information | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | Hirudo verbana | ? | - |
? | |
additional information | the destabilase-lysozyme is a bifunctional enzyme, which combines isopeptidase and lysozyme activities | Hirudo orientalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
destabilase-lysozyme | - |
Hirudo medicinalis |
destabilase-lysozyme | - |
Hirudo verbana |
destabilase-lysozyme | - |
Hirudo orientalis |
i-type lysozyme | - |
Hirudo medicinalis |
i-type lysozyme | - |
Hirudo verbana |
i-type lysozyme | - |
Hirudo orientalis |
invertebrate lysozyme | - |
Hirudo medicinalis |
invertebrate lysozyme | - |
Hirudo verbana |
invertebrate lysozyme | - |
Hirudo orientalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, substrate Micrococcus lysodeikticus cell walls | Hirudo medicinalis |
25 | - |
assay at, substrate Micrococcus lysodeikticus cell walls | Hirudo verbana |
25 | - |
assay at, substrate Micrococcus lysodeikticus cell walls | Hirudo orientalis |
42 | - |
assay at, substrate 4-methylumbelliferyl-tetra N-acetyl-beta-chitotetraoxide | Hirudo medicinalis |
42 | - |
assay at, substrate 4-methylumbelliferyl-tetra N-acetyl-beta-chitotetraoxide | Hirudo verbana |
42 | - |
assay at, substrate 4-methylumbelliferyl-tetra N-acetyl-beta-chitotetraoxide | Hirudo orientalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
assay at | Hirudo medicinalis |
5.2 | - |
assay at | Hirudo verbana |
5.2 | - |
assay at | Hirudo orientalis |
General Information | Comment | Organism |
---|---|---|
additional information | comparison of the levels of lysozyme, and peptidoglycan-lysing activity in salivary gland secretions of three species of the medicinal leech, overview | Hirudo medicinalis |
additional information | comparison of the levels of lysozyme, and peptidoglycan-lysing activity in salivary gland secretions of three species of the medicinal leech, overview | Hirudo verbana |
additional information | comparison of the levels of lysozyme, and peptidoglycan-lysing activity in salivary gland secretions of three species of the medicinal leech, overview | Hirudo orientalis |