Literature summary for 3.2.1.17 extracted from

Dang, L.P.; Fang, W.Z.; Li, Y.; Wang, Q.; Xiao, H.Z.; Wang, Z.Z.
Ionic liquid-induced structural and activity changes in hen egg white lysozyme (2013), Appl. Biochem. Biotechnol., 169, 290-300.

Activating Compound

Activating Compound	Comment	Organism
1,3-dimethylimidazolium iodine	50% activation at 5%	Gallus gallus
1-butyl-3-methylimidazolium bromide	below 20% activation at 5%	Gallus gallus
1-butyl-3-methylimidazolium chloride	below 10% activation at 5%	Gallus gallus
1-butyl-3-methylimidazolium tetrafluoroborate	below 20% activation at 5%	Gallus gallus
additional information	ionic liquids influence protein crystal morphology, size, polymorph, crystal quality, and modify solution properties, possible mechanisms, overview	Gallus gallus

Application

Application	Comment	Organism
food industry	due to increasing demands for natural food preservatives, lysozyme is increasingly important in food processing	Gallus gallus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in complex with 1-butyl-3-methylimidazoliumtetrafluoroborate, 1-butyl-3-methylimidazolium chloride, 1-butyl-3-methylimidazolium bromide, and 1,3-dimethylimidazolium iodine, 8% protein in 5% NaCl and 0.1 M sodium acetate, pH 4.5, ligands are injected into the protein solution. Supersaturated solutions are obtained by mixing protein stock solutions with precipitant solutions, 4°C, X-ray diffraction structure determination and analysis	Gallus gallus

Crystallization (Comment)

Organism

purified enzyme in complex with 1-butyl-3-methylimidazoliumtetrafluoroborate, 1-butyl-3-methylimidazolium chloride, 1-butyl-3-methylimidazolium bromide, and 1,3-dimethylimidazolium iodine, 8% protein in 5% NaCl and 0.1 M sodium acetate, pH 4.5, ligands are injected into the protein solution. Supersaturated solutions are obtained by mixing protein stock solutions with precipitant solutions, 4°C, X-ray diffraction structure determination and analysis

Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Gallus gallus	-
egg white	-	Gallus gallus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	lysis of Micrococcus lysodeikticus	Gallus gallus	?	-	?

Subunits

Subunits	Comment	Organism
More	Fourier transform infrared spectroscopy reveals that alpha-helix and beta-sheet are the major secondary structures for lysozyme	Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.3	-	assay at	Gallus gallus