Literature summary for 3.2.1.17 extracted from
Park, S.; Yoe, S.
Overexpression and purification of recombinant lysozyme from Agrius convolvuli expressed as inclusion body in Escherichia coli (2012), Anim. Cells Sys., 16, 455-461.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
overexpression of GST-tagged enzyme in inclusion body in Escherichia coli with induction of the fusion protein |
Agrius convolvuli |
Organism
Organism |
UniProt |
Comment |
Textmining |
Agrius convolvuli |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant GST-tagged enzyme from Escherichia coli inclusion bodies by solubilization and refolding, GST-tag cleavage through thrombin, and anion exchange chromatography and RPLC gel filtration |
Agrius convolvuli |
Renatured (Commentary)
Renatured (Comment) |
Organism |
recombinant GST-tagged enzyme in inclusion bodies is resolubilized in solubilization buffer and dialyzed in refolding buffer |
Agrius convolvuli |
General Information
General Information |
Comment |
Organism |
physiological function |
purified refolded recombinant enzyme exhibits antibacterial activity against Bacillus megaterium and Micrococcus luteus |
Agrius convolvuli |