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Literature summary for 3.2.1.17 extracted from

  • Yang, B.; Wang, J.; Tang, B.; Liu, Y.; Guo, C.; Yang, P.; Yu, T.; Li, R.; Zhao, J.; Zhang, L.; Dai, Y.; Li, N.
    Characterization of bioactive recombinant human lysozyme expressed in milk of cloned transgenic cattle (2011), PLoS ONE, 6, e17593.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme in transgenic cattle, Bos taurus, as secreted enzyme in milk using the pBC2-HLY-NEOR transgene vector Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from transgenic cow milk, from which fat and casein has been removed for purification of the enzyme, by cation exchange chromatography and gel filtration to over 95% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
Homo sapiens
-

Subunits

Subunits Comment Organism
? x * 14679.14, mass spectrometry Homo sapiens
More peptide mass fingerprinting of recombinant enzymes, overview Homo sapiens

Synonyms

Synonyms Comment Organism
HLZ
-
Homo sapiens
muramidase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant enzyme Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified recombinant enzyme, 45 min, 95% activity remaining Homo sapiens
100
-
purified recombinant enzyme, 10 min, 50% activity remaining Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 the optimal pH of rHLZ varies with salt concentration of the buffer, recombinant enzyme Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, recombinant enzyme Homo sapiens

General Information

General Information Comment Organism
additional information the modified bovine milk is a possible substitute for human milk Homo sapiens