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Literature summary for 3.2.1.17 extracted from

  • Ito, L.; Shiraki, K.; Yamaguchi, H.
    Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme (2010), Acta Crystallogr. Sect. F, 66, 750-754.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
pure enzyme, hanging drop vapour diffusion method, 50 or 150 mg/ml enzyme in 0.1 M sodium acetate, pH 4.5, sodium phosphate, pH 6.5, or Tris-HCl, pH 8.5, mixing of 0.0015 ml of protein solution and 0.0015 ml of reservoir solution, equilibration against 0.5 ml of reservoir solutiom, 20°C, crystallization method evaluation using Gly, Ser, Asp, Glu, Arg, ornithine, Lys and glycine ethyl ester as precipitants at pH 4.5, 6.5 and 8.5, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation purified Gallus gallus
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egg white
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Gallus gallus
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