Crystallization (Comment) | Organism |
---|---|
crystal structure of mutant N44Q/N47Q/N49Q/N68Q/N103Q is substantially identical to that of the wild type, and the substitutions of Asn to Gln are appropriate for the folding and structural analyses of this protein | Canis lupus familiaris |
Protein Variants | Comment | Organism |
---|---|---|
N44Q/N47Q/N49Q/N103Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites | Canis lupus familiaris |
N44Q/N47Q/N49Q/N68Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites | Canis lupus familiaris |
N44Q/N47Q/N49Q/N68Q/N103Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites. Decrease in lytic activity by a factor 20 compared with wild-type | Canis lupus familiaris |
N44Q/N47Q/N68Q/N103Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites | Canis lupus familiaris |
N44QN49Q/N68Q/N103Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites | Canis lupus familiaris |
N47Q/N49Q/N68Q/N103Q | mutant construcuted for study of charge heterogeneity caused by asparaginyl deamidation. Residues Asn 44, 47, 49, and 68 are the deamidation sites | Canis lupus familiaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
canine milk lysozyme exhibits charge heterogeneity after sample purification. Four Asn residues deamidate rapidly under mild conditions of pH 8.0 and 30°C. One Asn residue, which is stable in the native state, is labile to deamidation in the unfolded state | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Canis lupus familiaris | - |