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Literature summary for 3.2.1.17 extracted from

  • Matsushita, I.; Yanase, H.
    A novel thermophilic lysozyme from bacteriophage phiIN93 (2008), Biochem. Biophys. Res. Commun., 377, 89-92.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by 2-mercaptoethanol Thermus virus IN93

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, SDS-PAGE, x * 33141, calculated Thermus virus IN93
33141
-
x * 33000, SDS-PAGE, x * 33141, calculated Thermus virus IN93

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus virus IN93 enzyme lyses specifically Thermus aquaticus cells, with 79% activity on Thermus fhermophilus HB8 and 76% activity on Thermus filífformis ?
-
?
additional information Thermus virus IN93 phiIN93 enzyme lyses specifically Thermus aquaticus cells, with 79% activity on Thermus fhermophilus HB8 and 76% activity on Thermus filífformis ?
-
?

Organism

Organism UniProt Comment Textmining
Thermus virus IN93 Q859R8 phiIN93
-
Thermus virus IN93 phiIN93 Q859R8 phiIN93
-

Purification (Commentary)

Purification (Comment) Organism
from lysates of phage grown on Thermus aquaticus TZ2 Thermus virus IN93

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31050
-
pH 7.0, 70°C Thermus virus IN93

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme lyses specifically Thermus aquaticus cells, with 79% activity on Thermus fhermophilus HB8 and 76% activity on Thermus filífformis Thermus virus IN93 ?
-
?
additional information enzyme lyses specifically Thermus aquaticus cells, with 79% activity on Thermus fhermophilus HB8 and 76% activity on Thermus filífformis Thermus virus IN93 phiIN93 ?
-
?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE, x * 33141, calculated Thermus virus IN93

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
1 h, 38.6% residual activity in absence of 2-mercaptoethanol, 93.8% residual activity in presence of 2-mercaptoethanol Thermus virus IN93