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Literature summary for 3.2.1.17 extracted from

  • Fokine, A.; Miroshnikov, K.A.; Shneider, M.M.; Mesyanzhinov, V.V.; Rossmann, M.G.
    Structure of the bacteriophage phi KZ lytic transglycosylase gp144 (2008), J. Biol. Chem., 283, 7242-7250.
    View publication on PubMed

Application

Application Comment Organism
medicine the structure of gp144 might be useful for the development of new antibacterial reagents for preventing and treating Pseudomonas aeruginosa infections Phikzvirus phiKZ

Cloned(Commentary)

Cloned (Comment) Organism
into the pQE-30 vector for expression in Escherichia coli cells Phikzvirus phiKZ

Crystallization (Commentary)

Crystallization (Comment) Organism
the x-ray structure of the lytic transglycosylase gp144 is determined to 2.5 A resolution, in complex with chitotetraose, (N-acetylglucosamine)4, to 2.6 A resolution Phikzvirus phiKZ

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28800
-
calculated molecular mass Phikzvirus phiKZ

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Phikzvirus phiKZ cleaves the glycosidic linkage between N-acetylmuramoyl and N-acetylglucosaminyl residues additional information formation of a 1,6-anhydromuramoyl product ?

Organism

Organism UniProt Comment Textmining
Phikzvirus phiKZ Q8SD18 Pseudomonas bacteriophage
-

Purification (Commentary)

Purification (Comment) Organism
using a HisTrap HP column Phikzvirus phiKZ

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleaves the glycosidic linkage between N-acetylmuramoyl and N-acetylglucosaminyl residues Phikzvirus phiKZ additional information formation of a 1,6-anhydromuramoyl product ?

Synonyms

Synonyms Comment Organism
gp144
-
Phikzvirus phiKZ
phiKZ endolysin
-
Phikzvirus phiKZ
transglycosylase
-
Phikzvirus phiKZ