Application | Comment | Organism |
---|---|---|
agriculture | Lys411H is the first lysozyme active against both Xanthomonas and Stenotrophomonas maltophilia. This suggests that Lys411 can be a candidate to be developed into a therapeutic agent for treating Stenotrophomonas maltophilia infections, in addition to the potential use in control of the plant diseases caused by Xanthomonas | Xanthomonas phage Xo411 |
Cloned (Comment) | Organism |
---|---|
the His-tagged protein Lys411H is expressed in Escherichia coli mostly in inclusion bodies | Xanthomonas phage Xo411 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 1 mM | Xanthomonas phage Xo411 | |
EDTA | at 10 and 20 mM causes 15% and 43% reduction of the enzyme activity | Xanthomonas phage Xo411 | |
FeCl3 | 1 mM | Xanthomonas phage Xo411 | |
NiCl | 1 mM | Xanthomonas phage Xo411 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1.0 mM, activity is increased by 54% | Xanthomonas phage Xo411 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas phage Xo411 | A1BPG9 | bacteriophage of Xanthomonas oryzae pv. oryzae | - |
Purification (Comment) | Organism |
---|---|
the insoluble protein is solubilized in urea and purified by passing through a His-bind column, and the lytic activity is then restored by a refolding process | Xanthomonas phage Xo411 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
7400 U/mg. One unit of enzyme is defined as the amounts that caused the decrease of 0.001 units of absorbance at 550 nM per min | Xanthomonas phage Xo411 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | the enzyme is active against both Xanthomonas and Stenotrophomonas maltophilia. Only a minor portion of the Escherichia coli cells is lysed. Cells of Micococcus lysodeikticus, Bacillus subtilis, Agrobacterium tumefaciens, and Psuedomonas fluorescens exhibit no signifiant lysis | Xanthomonas phage Xo411 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C | Xanthomonas phage Xo411 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: maximal activity, 45°C: about 90% of maximal activity, 50°C: about 40% of maximal activity | Xanthomonas phage Xo411 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
10 min, 3% loss of activity | Xanthomonas phage Xo411 |
30 | - |
10 min, 14% loss of activity | Xanthomonas phage Xo411 |
40 | - |
10 min, 25% loss of activity | Xanthomonas phage Xo411 |
50 | - |
10 min, 30% loss of activity | Xanthomonas phage Xo411 |
55 | - |
10 min, 66% loss of activity | Xanthomonas phage Xo411 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C | Xanthomonas phage Xo411 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | pH 6.0: about 60% of maximal activity, pH 7.0: about 55% of maximal activity | Xanthomonas phage Xo411 |