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Literature summary for 3.2.1.17 extracted from

  • Lee, C.N.; Lin, J.W.; Chow, T.Y.; Tseng, Y.H.; Weng, S.F.
    A novel lysozyme from Xanthomonas oryzae phage varphiXo411 active against Xanthomonas and Stenotrophomonas (2006), Protein Expr. Purif., 50, 229-237.
    View publication on PubMed

Application

Application Comment Organism
agriculture Lys411H is the first lysozyme active against both Xanthomonas and Stenotrophomonas maltophilia. This suggests that Lys411 can be a candidate to be developed into a therapeutic agent for treating Stenotrophomonas maltophilia infections, in addition to the potential use in control of the plant diseases caused by Xanthomonas Xanthomonas phage Xo411

Cloned(Commentary)

Cloned (Comment) Organism
the His-tagged protein Lys411H is expressed in Escherichia coli mostly in inclusion bodies Xanthomonas phage Xo411

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 1 mM Xanthomonas phage Xo411
EDTA at 10 and 20 mM causes 15% and 43% reduction of the enzyme activity Xanthomonas phage Xo411
FeCl3 1 mM Xanthomonas phage Xo411
NiCl 1 mM Xanthomonas phage Xo411

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1.0 mM, activity is increased by 54% Xanthomonas phage Xo411

Organism

Organism UniProt Comment Textmining
Xanthomonas phage Xo411 A1BPG9 bacteriophage of Xanthomonas oryzae pv. oryzae
-

Purification (Commentary)

Purification (Comment) Organism
the insoluble protein is solubilized in urea and purified by passing through a His-bind column, and the lytic activity is then restored by a refolding process Xanthomonas phage Xo411

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
7400 U/mg. One unit of enzyme is defined as the amounts that caused the decrease of 0.001 units of absorbance at 550 nM per min Xanthomonas phage Xo411

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan + H2O the enzyme is active against both Xanthomonas and Stenotrophomonas maltophilia. Only a minor portion of the Escherichia coli cells is lysed. Cells of Micococcus lysodeikticus, Bacillus subtilis, Agrobacterium tumefaciens, and Psuedomonas fluorescens exhibit no signifiant lysis Xanthomonas phage Xo411 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C Xanthomonas phage Xo411

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: maximal activity, 45°C: about 90% of maximal activity, 50°C: about 40% of maximal activity Xanthomonas phage Xo411

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
10 min, 3% loss of activity Xanthomonas phage Xo411
30
-
10 min, 14% loss of activity Xanthomonas phage Xo411
40
-
10 min, 25% loss of activity Xanthomonas phage Xo411
50
-
10 min, 30% loss of activity Xanthomonas phage Xo411
55
-
10 min, 66% loss of activity Xanthomonas phage Xo411

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C Xanthomonas phage Xo411

pH Range

pH Minimum pH Maximum Comment Organism
6 7 pH 6.0: about 60% of maximal activity, pH 7.0: about 55% of maximal activity Xanthomonas phage Xo411