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Literature summary for 3.2.1.17 extracted from

  • Altintas, E.B.; Tuezmen, N.; Candan, N.; Denizli, A.
    Use of magnetic poly(glycidyl methacrylate) monosize beads for the purification of lysozyme in batch system (2007), J. Chromatogr. B, 853, 105-113.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Gallus gallus
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Purification (Commentary)

Purification (Comment) Organism
hydrophobic affinity ligand L-tryptophan immobilized magnetic poly(glycidyl methacrylate) [m-poly(GMA)] beads in monosize form (0.0016 mM in diameter) are used for the affinity purification of lysozyme from chicken egg white. The chemisorption processes can be the rate-limiting step in the adsorption process. After 10 adsorption-elution cycles, m-poly(GMA)-L-tryptophan beads can be used without significant loss in lysozyme adsorption capacity Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
egg white
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Gallus gallus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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one unit lysozyme activity is defined as the amount of enzyme causing a decrase of 0.001 optical density value per minute at 25°C and pH 7.0. Specific activity of the eluted lysozyme (62580 U/mg) is higher than that obtained with a commercially available pure lysozyme Sigma (60000 U/mg) Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan + H2O Micrococcus lysodeikticus cells Gallus gallus ?
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