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Literature summary for 3.2.1.17 extracted from

  • Kawamura, S.; Ohno, K.; Ohkuma, M.; Chijiiwa, Y.; Torikata, T.
    Experimental verification of the crucial roles of Glu73 in the catalytic activity and structural stability of goose type lysozyme (2006), J. Biochem., 140, 75-85.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Struthio camelus

Protein Variants

Protein Variants Comment Organism
E73A no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 3.2°C as compared to wild-type value Struthio camelus
E73D detectable level of activity, but lytic activity against Micrococcus lysodeikticus is drastically reduced as compared to wild-type activity. Decrease in Tm-value is 6.1°C as compared to wild-type value Struthio camelus
E73Q no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 4.0°C as compared to wild-type value Struthio camelus

Organism

Organism UniProt Comment Textmining
Struthio camelus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Struthio camelus

Renatured (Commentary)

Renatured (Comment) Organism
when the thermal denaturation curves are analyzed in the absence of guanidine hydrochloride, the reversibility calculated is 76% for the wild-type enzyme, 80% for E73D, 80% for E73Q and 79% for E73A. In the presence of 0.5 M guanidine hydrochloride, the reversibility of the thermal unfolding for the wild type and mutant proteins is more than 95% Struthio camelus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O wild-type protein hydrolyzes N,N',N'',N''',N''''-pentaacetylchitopentaose almost completely on 140 min reaction. N,N',N'',N'''-tetraacetylchitotetraose is is hydrolyzed mainly to N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose with much less cleavage into GlcNAc + N,N',N'',N'''-tetraacetylchitotetraose Struthio camelus N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose
-
?
peptidoglycan + H2O lytic activity against Micrococcus lysodeikticus Struthio camelus ?
-
?

Synonyms

Synonyms Comment Organism
goose type lysozyme
-
Struthio camelus
OEL
-
Struthio camelus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
Tm-value for mutant enzyme E73S is 54.5°C Struthio camelus
57
-
Tm-value for mutant E73A is 57.4°C. Tm-value for mutant E73Q is 56.6°C Struthio camelus
61
-
Tm-value for wild-type enzyme is 60.6°C Struthio camelus