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Literature summary for 3.2.1.17 extracted from

  • Manas, P.; Munoz, B.; Sanz, D.; Condon, S.
    Inactivation of lysozyme by ultrasonic waves under pressure at different temperatures (2006), Enzyme Microb. Technol., 39, 1177-1182.
    View publication on PubMed

Application

Application Comment Organism
synthesis the extracellular pH-sensitive glycosylation system can be used to obtain bioactive and surface functional neoglycoproteins Gallus gallus

Cloned(Commentary)

Cloned (Comment) Organism
a large polymannose (Man310GlcNAc2) chain-linked lysozyme is predominantly expressed in Saccharomyces cerevisiae accompanied by small amounts of a core-type oligomannose chain (Man14GlcNAc2)-linked lysozyme in the yeast medium where the extracellular pH is kept at 3.5 or above, while an oligomannose chain lysozyme is preferentially expressed in the yeast medium where the pH is less than 3 Gallus gallus

Protein Variants

Protein Variants Comment Organism
additional information the lytic activities of the oligomannosyl lysozyme (a core-type oligomannose chain (Man14GlcNAc2)-linked lysozyme and) and the polymannosyl lysozymes (a large polymannose (Man310GlcNAc2) chain-linked lysozyme) are 70.4% and 5.1%, respectively, of the wild-type lysozyme when Micrococcus lysodeikticus cells are used as the substrate. The enzymatic activity of the oligomannosyl lysozyme is totally conserved for the glycolysis assay with a soluble substrate, glycol chitin, whereas that of the polymannosyl lysozyme is not. After heating the sample up to 95 °C at pH 7.0 where no visible protein coagulation is observed, thermostability of the enzymatic activity of the oligomannosyl lysozyme is drastically improved with more than 60% of residual lytic activity. Emulsifying properties of the protein also are highly improved by the oligomannosylation, in which the emulsifying activity is 3.2 times higher than that of the wild-type protein. Corresponding to the increase of the surface functionalities, the surface tension of the oligomannosyl protein exhibits a significantly lower value compared to that of the wild-type Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
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-
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Source Tissue

Source Tissue Comment Organism Textmining
egg white
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Gallus gallus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycol chitin + H2O
-
Gallus gallus ?
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?
peptidoglycan + H2O Micrococcus lysodeikticus cells Gallus gallus ?
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?