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Literature summary for 3.2.1.17 extracted from

  • Li, S.; Li, B.; Fei, Y.; Jiang, D.; Sheng, Y.; Sun, Y.; Zhang, J.
    Exon grafting yields a "two active-site" lysozyme (2007), Biochem. Biophys. Res. Commun., 358, 997-1001.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information a strategy to engineer an additional active site for human lysozyme: grafting the entire human lysozyme exon 2, which encodes the catalytically competent domain, into the gene at a position corresponding to an exposed loop region in the translated protein. Exon 2 grafting creates a novel lysozyme with twice the activity of the wild type enzyme, equal activity came from each of the two active sites. The thermal stability and pH-stability of wild-type and two-active site lysozyme is similar Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Storage Stability

Storage Stability Organism
no significant loss of the activity after 6 months of frozen storage for both wild-type and two-active site lysozyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan + H2O lytic activity against Micrococcus lysodeikticus Homo sapiens ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
wild-type enzyme, lytic activity against Micrococcus lysodeikticus Homo sapiens
6.5
-
two-active site lysozyme, lytic activity against Micrococcus lysodeikticus Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.4 7.2 wild-type and two-active site lysozyme both retain over 80% activity as determined by assaying the lysis of Micrococcus lysodeikticus cells over a pH range of 5.3 to 7.2 Homo sapiens