Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a strategy to engineer an additional active site for human lysozyme: grafting the entire human lysozyme exon 2, which encodes the catalytically competent domain, into the gene at a position corresponding to an exposed loop region in the translated protein. Exon 2 grafting creates a novel lysozyme with twice the activity of the wild type enzyme, equal activity came from each of the two active sites. The thermal stability and pH-stability of wild-type and two-active site lysozyme is similar | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
no significant loss of the activity after 6 months of frozen storage for both wild-type and two-active site lysozyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | lytic activity against Micrococcus lysodeikticus | Homo sapiens | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
wild-type enzyme, lytic activity against Micrococcus lysodeikticus | Homo sapiens |
6.5 | - |
two-active site lysozyme, lytic activity against Micrococcus lysodeikticus | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 7.2 | wild-type and two-active site lysozyme both retain over 80% activity as determined by assaying the lysis of Micrococcus lysodeikticus cells over a pH range of 5.3 to 7.2 | Homo sapiens |