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Literature summary for 3.2.1.17 extracted from

  • Pooart, J.; Torikata, T.; Araki, T.
    Enzymatic properties of rhea lysozyme (2005), Biosci. Biotechnol. Biochem., 69, 103-112.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Anser sp.
-
-
-
Casuarius casuarius
-
-
-
Rhea americana
-
-
-
Struthio camelus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
Rhea americana
-
egg white
-
Anser sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc)6 + H2O
-
Rhea americana (GlcNAc)3 and smaller amounts of (GlcNAc)2 and (GlcNAc)4 endo-splitting, hydrolyzes preferentially the third glycosidic linkage from the nonreducing end ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Rhea americana ?
-
?
peptidoglycan + H2O lyophilized cell wall of Micrococcus luteus Anser sp. ?
-
?
peptidoglycan + H2O lyophilized cell wall of Micrococcus luteus Struthio camelus ?
-
?
peptidoglycan + H2O lyophilized cell wall of Micrococcus luteus Rhea americana ?
-
?
peptidoglycan + H2O lyophilized cell wall of Micrococcus luteus Casuarius casuarius ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40 substrate: lyophilized cell wall of Micrococcus luteus Struthio camelus
40
-
substrate: lyophilized cell wall of Micrococcus luteus Rhea americana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 85% of maximal activity, 60°C: about 75% of maximal activity, substrate: lyophilized cell wall of Micrococcus luteus Struthio camelus
20 60 20°C: about 95% of maximal activity, 60°C: about 65% of maximal activity, substrate: lyophilized cell wall of Micrococcus luteus Rhea americana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 10 min, stable Struthio camelus
20 30 10 min, stable Rhea americana
40
-
10 min, about 20% loss of activity Rhea americana
40
-
10 min, about 15% loss of activity Struthio camelus
50
-
10 min, about 30% loss of activity Struthio camelus
60
-
10 min, about 25% loss of activity Rhea americana
60 70 10 min, about 40% loss of activity Struthio camelus
70
-
10 min, about 30% loss of activity Rhea americana
80
-
10 min, about 45% loss of activity Rhea americana
80
-
10 min, about 50% loss of activity Struthio camelus
90
-
about 60% loss of activity after 60 min, about 80% loss of activity after180 min Struthio camelus
90
-
about 60% loss of activity after 60 min, about 80% loss of activity after180 min Rhea americana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
substrate: (GlcNAc)5 Rhea americana
5
-
substrate: lyophilized cell wall of Micrococcus luteus Casuarius casuarius
5.5
-
substrate: lyophilized cell wall of Micrococcus luteus Anser sp.
6
-
substrate: lyophilized cell wall of Micrococcus luteus Struthio camelus
6
-
substrate: lyophilized cell wall of Micrococcus luteus Rhea americana

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3: about 65% of maximal activity, pH 5: about 40% of maximal activity, substrate: (GlcNAc)5 Rhea americana
4.5 5.5 pH 4.5: about 60% of maximal activity, pH 5.5: about 70% of maximal activity, substrate: lyophilized cell wall of Micrococcus luteus Casuarius casuarius
5 6.5 pH 5: about 45% of maximal activity, pH 6.5: about 50% of maximal activity, substrate: lyophilized cell wall of Micrococcus luteus Struthio camelus
5 6.5 pH 5: about 60% of maximal activity, pH 6.5: about 50% of maximal activity, substrate: lyophilized cell wall of Micrococcus luteus Anser sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 37°C, 4 h, stable Struthio camelus
3 10 37°C, 4 h, stable Rhea americana